PARC Antibody
| Code | Size | Price |
|---|
| PSI-3171-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3171-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
PARC Antibody: PARC, H7AP1, KIAA0708, PARC, Cullin-9, UbcH7-associated protein 1, CUL-9
Application Note:
PARC antibody can be used for the detection of PARC by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PARC Antibody: The continued localization of p53 to the nucleus is essential for its function as a tumor suppressor. PARC, a large, Parkin-like ubiquitin ligase has recently been identified as a cytoplasmic anchor protein in p53-associated protein complexes. In the absence of stress, PARC inactivation results in nuclear localization of p53 and activation of p53-dependent apoptosis, while overexpression of this protein promoted cytoplasmic sequestration of p53. Surprisingly, PARC knockout mice were viable and exhibited no obvious phenotype, suggesting that other proteins, such as the highly related cullin family of E3 ubiquitin ligases, may perform similar functions in the absence of PARC. Additionally, it has been suggested that p53 binding to PARC may serve to control PARC function.
Background References:
- Nikolaev AY, Li M, Puskas N, et al. Parc: a cytoplasmic anchor for p53. Cell 2003; 112:29-40.
- Skaar JR, Arai T, and DeCaprio JA. Dimerization of CUL7 and PARC is not required for all CUL7 functions and mouse development. Mol. Cell. Biol. 2005; 25:5579-89.
Buffer:
PARC Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PARC antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PARC.
The immunogen is located within the last 50 amino acids of PARC.
The immunogen is located within the last 50 amino acids of PARC.
NCBI Gene ID #:
23113
NCBI Official Name:
cullin 9
NCBI Official Symbol:
CUL9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055904
Protein GI Number:
24307991
Purification:
PARC Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
Swissprot #:
Q8IWT3
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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