ATM Antibody
| Code | Size | Price |
|---|
| PSI-3945-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3945-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ATM Antibody: AT1, ATA, ATC, ATD, ATE, ATDC, TEL1, TELO1, Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated
Application Note:
ATM antibody can be used for detection of ATM by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ATM Antibody: DNA double strand breaks represent a major threat to an organism's genome. Eukaryotic cells have developed mechanisms that sense the presence this damage and initiate suitable responses that can include DNA repair, cell cycle delay, and programmed cell death. The ATM (mutated in Ataxia-Telangiectasia) protein kinase is activated following the formation of DNA double strand breaks, phosphorylating p53 and another kinase CHK2. This initiates a signaling cascade leading to the phosphorylation and inhibition of Cdc25, ultimately preventing cell cycle progression. In some cell types, such as the hemapoietic system, this leads to apoptosis instead of cell cycle arrest. Multiple isoforms of ATM are known to exist.
Background References:
- Cahill D, Connor B, and Carney JP. Mechanisms of eukaryotic DNA double strand break repair. Front. Biosci. 2006?; 11?:1958-76.
- Dasika GK, Lis SC, Zhao S, et al. DNA damage-induced cell cycle checkpoints and DNA strand break repair in development and tumorigenesis. Oncogene 1999; 18:7883-99.
Buffer:
ATM Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (86%), Mouse: (71%)
Immunogen:
ATM antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human ATM.
The immunogen is located within amino acids 2970 - 3020 of ATM.
The immunogen is located within amino acids 2970 - 3020 of ATM.
NCBI Gene ID #:
472
NCBI Official Name:
ataxia telangiectasia mutated
NCBI Official Symbol:
ATM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAB65827
Protein GI Number:
2304971
Purification:
ATM Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Cancer
Swissprot #:
Q13315
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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