Toso Antibody

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ProSci
Product Code: PSI-2273
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2273-0.02mg0.02mg£150.00
Quantity:
PSI-2273-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of TOSO in K562 cell lysate with TOSO antibody at (A) 1 and (B) 2 μg/mL.
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Western blot analysis of TOSO in K562 cell lysate with TOSO antibody at (A) 1 and (B) 2 μg/mL.

Documents

Further Information

Additional Names:
Toso Antibody: FCMR, TOSO, Fas apoptotic inhibitory molecule 3, Regulator of Fas-induced apoptosis Toso
Application Note:
Toso antibody can be used for detection of Toso by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
Toso Antibody: Apoptosis is an important process by which normal tissue homeostasis and function are maintained. One of the major signals that regulate this process is mediated by the activation of the Fas receptor by its ligand. This leads to the formation of a Fas-associated death domain (FADD)- containing death-inducing signaling complex and the activation of caspase-8, which in turn activates downstream effector caspases, such as caspase-3 and -7. Recent experiments have shown that overexpression of Toso, a novel regulator of Fas-induced apoptosis in lymphoid cells, in Jurkat cells as well as transgenic mice render these cells resistant to Fas-induced apoptosis but not to TRAIL-induced apoptosis. Furthermore, Toso was found to associate with FADD, suggesting that Toso functions by disrupting the formation of the death-inducing signaling complex.
Background References:
  • Curtin JF and Cotter TG. Live and let die: regulatory mechanisms in Fas-mediated apoptosis. Cell Signal. 2003; 15:983-92.
  • Hitoshi Y, Lorens J, Kitada S-I, et al. Toso, a cell surface, specific regulator of Fas-induced apoptosis in T cells. Immunity 1998; 8:461-71.
  • Song Y and Jacob CO. The mouse cell surface protein Toso regulates Fas/Fas ligand-induced apoptosis through its binding to Fas-associated death domain. J. Biol. Chem.2005; 280:9618-26.
Buffer:
Toso Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Toso antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human Toso.

The immunogen is located within the first 50 amino acids of Toso.
NCBI Gene ID #:
9214
NCBI Official Name:
Fas apoptotic inhibitory molecule 3
NCBI Official Symbol:
FAIM3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 43 kDa

Observed: 41 kDa
Protein Accession #:
NP_005440
Protein GI Number:
4885641
Purification:
Toso Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
O60667
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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