IKAP Antibody

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ProSci
Product Code: PSI-2337
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2337-0.02mg0.02mg£150.00
Quantity:
PSI-2337-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of IKAP in A-20 cell lysate with IKAP antibody at in (A) 0.5, and (B) 1 μg/mL.
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Immunocytochemistry of IKAP in A-20 cells with IKAP antibody at 1 μg/mL.
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Immunofluorescence of IKAP in A20 cells with IKAP antibody at 20 μg/mL.
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Western blot analysis of IKAP in A-20 cell lysate with IKAP antibody at in (A) 0.5, and (B) 1 μg/mL.
Immunocytochemistry of IKAP in A-20 cells with IKAP antibody at 1 μg/mL.
Immunofluorescence of IKAP in A20 cells with IKAP antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
IKAP Antibody: FD, DYS, ELP1, IKAP, IKI3, TOT1, Elongator complex protein 1, IkappaB kinase complex-associated protein
Application Note:
IKAP antibody can be used for detection of IKAP by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
IKAP Antibody: IKAP was initially identified as a scaffold protein of the IκB kinase complex that could bind to IKKα, IKKβ, NF-κB, and the NF-κB-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD.
Background References:
  • Cohen L, Henzel WJ, and Baeuerle PA. IKAP is a scaffold protein of the IkB kinase complex. Nature 1998; 395:292-6.
  • Krappmann D, Hatada EN, Tegethoff S, et al. The I kappa B kinase (IKK) complex is tripartite and contains IKK gamma but not IKAP as a regular component. J. Biol. Chem. 2000; 275:29779-87.
  • Anderson SL, Coli R, Daly IW, et al. Familial dysautonomia is caused by mutations of the IKAP gene. Am. J. Hum. Genet. 2001; 68:753-8.
  • Hawkes NA, Otero G, Winkler GS, et al. Purification and characterization of the human elongator complex. J. Biol. Chem. 2002; 277:3047-52.
Buffer:
IKAP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (88%), Rat: (75%)
Immunogen:
IKAP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human IKAP.

The immunogen is located within the last 50 amino acids of IKAP.
NCBI Gene ID #:
8518
NCBI Official Name:
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein
NCBI Official Symbol:
IKBKAP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 134, 147 kDa

Observed: 105, 145 kDa
Protein Accession #:
AAC64258
Protein GI Number:
3757822
Purification:
IKAP Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
At least two isoforms of IKAP are known two exist, this antibody will detect both isoforms.
Swissprot #:
O95163
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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