Slug Antibody

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ProSci
Product Code: PSI-3957
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3957-0.02mg0.02mg£150.00
Quantity:
PSI-3957-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of Slug in human kidney cell lysate with Slug antibody at in (A) 1 and (B) 2 μg/mL.
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Western blot analysis of Slug in human kidney cell lysate with Slug antibody at in (A) 1 and (B) 2 μg/mL.

Documents

Further Information

Additional Names:
Slug Antibody: SLUG, WS2D, SLUGH1, SNAIL2, SLUG, SLUGH, Zinc finger protein SNAI2, Neural crest transcription factor Slug
Application Note:
Slug antibody can be used for detection of Slug by Western blot at 1 to 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
Slug Antibody: Slug, a member of the Snail family of C2H2-type zinc finger transcription factors, was initially identified to be involved in epithelial-mesenchymal transitions as well as the formation of the neural tube during vertebrate embryogenesis. Like Snail, Slug transcription can be induced by growth factors such as FGF, BMP, and TGF-beta. Once expressed, Slug will bind E-box regions of promoters and repress transcription of genes such as E-cadherin and Claudin-1. More recently, its expression in breast, esophogeal, and colorectal carcinomas has been correlated with poor prognosis for survival. Furthermore, Slug can protect hemapoietic progenitor cells from radiation-induced apoptosis by repressing the p53-mediated transcription of Puma, a BH3-only antagonist of the anti-apoptotic members of the Bcl-2 family. Slug antibody has no cross-reactivity to Snail protein.
Background References:
  • Neito MA, Sargent MG, Wilkinson DG, et al. Control of cell behavior during vertebrate development by Slug, a zinc finger gene. Science 1994; 264:835-9.
  • Martinez-Estrada OM, Culleres A, Soriano FX, et al. The transcription factors Slug and Snail act as repressors of Claudin-1 expression in epithelial cells. Biochim J. 2006; 394:449-57.
  • Shioiri M, Shida T, Koda K, et al. Slug expression is an independent prognostic parameter for poor survival in colorectal carcinoma patients. Br. J. Cancer 2006; 94:1816-22.
  • Wu WS, Heinrichs S, Xu D, et al. Slug antagonizes p53-mediated apoptosis of hematopoietic progenitors by repressing puma. Cell 2005; 123:641-53.
Buffer:
Slug Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (100%)
Immunogen:
Slug antibody was raised against a 14 amino acid synthetic peptide from near the center of human Slug.

The immunogen is located within amino acids 70 - 120 of Slug.
NCBI Gene ID #:
6591
NCBI Official Name:
snail homolog 2 (Drosophila)
NCBI Official Symbol:
SNAI2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
O43623
Protein GI Number:
11134406
Purification:
Slug Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Cancer
Swissprot #:
O43623
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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