PEN2 Antibody

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ProSci
Product Code: PSI-3979
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3979-0.02mg0.02mg£150.00
Quantity:
PSI-3979-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of PEN2 in K562 cell lysate with PEN2 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
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Immunofluorescence of PEN2 in K562 cells with PEN2 antibody at 20 μg/mL.
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Western blot analysis of PEN2 in K562 cell lysate with PEN2 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunofluorescence of PEN2 in K562 cells with PEN2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
PEN2 Antibody: PEN2, PEN-2, MDS033, MSTP064, PEN2, Gamma-secretase subunit PEN-2, Presenilin enhancer protein 2
Application Note:
PEN2 antibody can be used for detection of PEN2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PEN2 Antibody: PEN2, in addition to presenilin, nicastrin, and APH-1 forms the gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease.
Background References:
  • Weihofen A and Martoglio B. Intramembrane-cleaving proteases: controlled liberation of proteins and bioactive peptides. Trends Cell Biol. 2003; 13:71-8.
  • Periz G and Fortini ME. Functional reconstitution of g-secretase through coordinated expression of presenilin, nicastrin, aph-1, and pen-2. J. Neurosci. Res. 2004; 77:309-22.
  • Selkoe DJ. The cell biology of b-amyloid precursor protein and presenilin in Alzheimer's disease. Trends Cell Biol. 1998; 8:447-53.
  • Selkoe SJ. Translating cell biology into therapeutic advances in Alzheimer's disease. Nature 1999; 399:A23-31
Buffer:
PEN2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
PEN2 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human PEN2.

The immunogen is located within the last 50 amino acids of PEN2.
NCBI Gene ID #:
55851
NCBI Official Name:
presenilin enhancer 2 homolog (C. elegans)
NCBI Official Symbol:
PSENEN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_758844
Protein GI Number:
28144920
Purification:
PEN2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
Q9NZ42
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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