SnoN Antibody

Contact us
ProSci
Product Code: PSI-2255
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2255-0.02mg0.02mg£150.00
Quantity:
PSI-2255-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of SnoN in A431 cell lysate with SnoN antibody at (A) 0.5 and (B) 1 μg/mL.
2 / 3
Immunohistochemistry of SnoN in mouse lung tissue with SnoN antibody at 5 μg/mL.
3 / 3
Immunofluorescence of SnoN in Mouse Lung cells with SnoN antibody at 20 μg/mL.
❮❯

Western blot analysis of SnoN in A431 cell lysate with SnoN antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of SnoN in mouse lung tissue with SnoN antibody at 5 μg/mL.
Immunofluorescence of SnoN in Mouse Lung cells with SnoN antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
SnoN Antibody: SNO, SnoA, SnoI, SnoN, SNO, Ski-like protein, Ski-related oncogene
Application Note:
SnoN antibody can be used for detection of SnoN by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
SnoN Antibody: TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells. There are at least four alternately spliced isoforms of SnoN; SnoN antibody will recognize all isoforms (SnoN, SnoN2, SnoI, and SnoA).
Background References:
  • Derynck R, Akhurst RJ, and Balmain A. TGF-β signaling in tumor suppression and cancer progression. Nat. Genet. 2001; 29:117-129.
  • Li Y, Turck CM, Teumer JK, et al. Unique sequence, Ski, in Sloan-Kettering avian retrovirus with properties of a new cell-derived oncogene. J. Virol. 1986; 57:1065-72.
  • Luo K. Ski and SnoN: negative regulators of TGF-β signaling. Curr. Op. Gen. Dev. 2004; 14:65-70.
  • Massague J and Wotton D. Transcriptional control by the TGF-β/Smad signaling system. EMBO J. 2000; 19:1745-54.
Buffer:
SnoN Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SnoN antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SnoN.

The immunogen is located within the first 50 amino acids of SnoN.
NCBI Gene ID #:
6498
NCBI Official Name:
SKI-like oncogene
NCBI Official Symbol:
SKIL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005405
Protein GI Number:
223029418
Purification:
SnoN Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Cancer
Swissprot #:
P12757
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
SnoN PeptidePSI-2255PProSci Summary Details