Ski Antibody
| Code | Size | Price |
|---|
| PSI-2259-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-2259-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Ski Antibody: SGS, SKV, Ski oncogene, Proto-oncogene c-Ski
Application Note:
SkiP antibody can be used for detection of Ski by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Ski Antibody: TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.
Background References:
- Derynck R, Akhurst RJ, and Balmain A. TGF-β signaling in tumor suppression and cancer progression. Nat. Genet. 2001; 29:117-129.
- Li Y, Turck CM, Teumer JK, et al. Unique sequence, ski, in Sloan-Kettering avian retrovirus with properties of a new cell-derived oncogene. J. Virol. 1986; 57:1065-72.
- Luo K. Ski and SnoN: negative regulators of TGF-β signaling. Curr. Op. Gen. Dev. 2004; 14:65-70.
- Massague J and Wotton D. Transcriptional control by the TGF-b/Smad signaling system. EMBO J. 2000; 19:1745-54.
Buffer:
Ski Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (100%), Horse: (100%), Mouse: (85%)
Immunogen:
Ski antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Ski.
The immunogen is located within the first 50 amino acids of Ski.
The immunogen is located within the first 50 amino acids of Ski.
NCBI Gene ID #:
6497
NCBI Official Name:
v-ski sarcoma viral oncogene homolog (avian)
NCBI Official Symbol:
SKI
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
P12755
Protein GI Number:
134517
Purification:
Ski Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Cancer
Swissprot #:
P12755
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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