SkiP Antibody

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ProSci
Product Code: PSI-2261
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2261-0.02mg0.02mg£150.00
Quantity:
PSI-2261-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of SkiP in mouse skeletal muscle tissue lysate with SkiP antibody at 0.5 μg/ml.
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Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.
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Western blot analysis of SkiP in mouse skeletal muscle tissue lysate with SkiP antibody at 0.5 μg/ml.
Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
SkiP Antibody: Bx42, SKIP, Prp45, SKIIP, PRPF45, NCOA-62, SNW domain-containing protein 1, Nuclear protein SkiP
Application Note:
SkiP antibody can be used for detection of SkiP by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SkiP Antibody: TGF-beta and the bone morphogenic proteins (BMPs) are key signaling proteins that regulate numerous cellular processes such as embryonic development and tumorigenesis. Both signal through the Smad protein family and are negatively regulated by Ski and SnoN, two related proto-oncoproteins. Ski functions by binding to the Smad proteins activated by TGF-beta and the (BMPs) and preventing their phosphorylation, inhibiting their ability to bind DNA and activate the transcription of downstream genes. SkiP was originally identified as a Ski-interacting protein and was later found to augment the signals induced by TGF-beta but inhibit transcription induced by BMP-2 in C2C12 cells, suggesting that SkiP is a key player in the signaling cascades inititated by TGF-beta and the BMP protein family.
Background References:
  • Derynck R, Akhurst RJ, and Balmain A. TGF-β signaling in tumor suppression and cancer progression. Nat. Genet. 2001; 29:117-129.
  • Li Y, Turck CM, Teumer JK, et al. Unique sequence, SkiP, in Sloan-Kettering avian retrovirus with properties of a new cell-derived oncogene. J. Virol. 1986; 57:1065-72.
  • Luo K. SkiP and SkiP: negative regulators of TGF-β signaling. Curr. Op. Gen. Dev. 2004; 14:65-70.
  • Massague J and Wotton D. Transcriptional control by the TGF-b/Smad signaling system. EMBO J. 2000; 19:1745-54.
Buffer:
SkiP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
SkiP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human SkiP.

The immunogen is located within the last 50 amino acids of SkiP.
NCBI Gene ID #:
22938
NCBI Official Name:
SNW domain containing 1
NCBI Official Symbol:
SNW1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 58, 62 kDa

Observed: 65 kDa?
Protein Accession #:
NP_036377
Protein GI Number:
6912676
Purification:
SkiP Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q13573
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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