PDCD4 Antibody

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ProSci
Product Code: PSI-3975
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3975-0.02mg0.02mg£150.00
Quantity:
PSI-3975-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of PDCD4 in A-20 cell lysate with PDCD4 antibody at (A) 0.5 and (B) 1μg/mL.
2 / 2
Immunohistochemistry of PDCD4 in mouse brain tissue with PDCD antibody at 2.5 μg/mL.
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Western blot analysis of PDCD4 in A-20 cell lysate with PDCD4 antibody at (A) 0.5 and (B) 1μg/mL.
Immunohistochemistry of PDCD4 in mouse brain tissue with PDCD antibody at 2.5 μg/mL.

Documents

Further Information

Additional Names:
PDCD4 Antibody: H731, H731, Programmed cell death protein 4, Neoplastic transformation inhibitor protein
Application Note:
PDCD4 antibody can be used for detection of PDCD4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
PDCD4 Antibody: Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.
Background References:
  • Jin Z and El Deiry WS. Overview of cell death signaling pathways. Cancer Biol. Ther. 2004; 4:139-63.
  • Shibihara K, Asano M, Ishida Y, et al. Isolation of a novel mouse gene MA-3 that is induced upon programmed cell death. Gene 1995; 166:297-301.
  • Goke R, Barth P, Schmidt A, et al. Programmed cell death 4 suppresses CDK1/cdc2 via induction of p21 (Waf1/Cip1). Am. J. Physiol. Cell Physiol. 2004; 287:C1541-6.
  • Yang HS, Jansen AP, Nair R, et al. The transformation suppressor Pdcd4 is a novel translation initiation factor 4A binding protein that inhibits translation. Mol. Cell Biol. 2003; 23:26-37.
Buffer:
PDCD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (100%)
Immunogen:
PDCD4 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human PDCD4.

The immunogen is located within the last 50 amino acids of PDCD4.
NCBI Gene ID #:
27250
NCBI Official Name:
programmed cell death 4 (neoplastic transformation inhibitor)
NCBI Official Symbol:
PDCD4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_663314
Protein GI Number:
21735598
Purification:
PDCD4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Cancer
Swissprot #:
Q53EL6
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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