TMP21 Antibody
| Code | Size | Price |
|---|
| PSI-3997-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3997-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
TMP21 Antibody: p23, TMP21, S31I125, Tmp-21-I, S31III125, P24(DELTA), Transmembrane emp24 domain-containing protein 10, 21 kDa transmembrane-trafficking protein
Application Note:
TMP21 antibody can be used for detection of TMP21 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TMP21 Antibody: TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate gamma-secretase activity.
Background References:
- Blunt R, Feick P, Puype M, et al. Tmp21 and p24A, two type I proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking. J. Biol. Chem.1996; 271:17183-9.
- Chen F, Hasegawa H, Schmitt-Ulms G, et al. TMP21 is a presenilin complex component that modulates γ-secretase but not ε-secretase activity. Nature 2006; 440:1208-12.
- Periz G and Fortini ME. Functional reconstitution of γ-secretase through coordinated expression of presenilin, nicastrin, aph-1, and pen-2. J. Neurosci. Res. 2004; 77:309-22.
- Selkoe DJ. The cell biology of β-amyloid precursor protein and presenilin in Alzheimer's disease. Trends Cell Biol. 1998; 8:447-53.
Buffer:
TMP21 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: (100%)
Immunogen:
TMP21 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human TMP21.
The immunogen is located within amino acids 160 - 210 of TMP21.
The immunogen is located within amino acids 160 - 210 of TMP21.
NCBI Gene ID #:
10972
NCBI Official Name:
transmembrane emp24-like trafficking protein 10 (yeast)
NCBI Official Symbol:
TMED10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAD31941
Protein GI Number:
4885697
Purification:
TMP21 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
P49755
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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