APH1 Antibody
| Code | Size | Price |
|---|
| PSI-4001-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4001-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
APH1 Antibody: APH-1, APH-1A, CGI-78, 6530402N02Rik, PSF, UNQ579/PRO1141, Gamma-secretase subunit APH-1A, Aph-1alpha, APH-1a
Application Note:
APH1 antibody can be used for detection of APH1 by Western blot at 0.5 - 2 μg/mL. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
APH1 Antibody: APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the gamma-secretase complex which cleave the amyloid precursor protein (APP) at what are known as the gamma- and epsilon-sites and can lead to the accumulation of the Amyloid beta peptide (Abeta) cleavage product that is associated with Alzheimer's disease. APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the gamma-secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed.
Background References:
- Goutte C, Tsunozaki M, Hale VA, et al. APH-1 is a multipass membrane protein essential for the Notch signaling pathway in Caenorhabditis elegans embryos. Proc. Natl. Acad. Sci. USA2002; 99:775-9.
- Periz G and Fortini ME. Functional reconstitution of γsecretase through coordinated expression of presenilin, nicastrin, aph-1, and pen-2. J. Neurosci. Res. 2004; 77:309-22.
- Selkoe DJ. The cell biology of βamyloid precursor protein and presenilin in Alzheimer's disease. Trends Cell Biol. 1998; 8:447-53.
- Ma G, Li T, Price DL, et al. APH-1a is the principal mammalian aph-1 isoform present in g-secretase complexes during embryonic development. Neuro. Dis. 2005; 25:192-8.
Buffer:
APH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
APH1 antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human APH1a.
The immunogen is located within amino acids 40 - 90 of APH1.
The immunogen is located within amino acids 40 - 90 of APH1.
NCBI Gene ID #:
51107
NCBI Official Name:
anterior pharynx defective 1 homolog A (C. elegans)
NCBI Official Symbol:
APH1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAH08732
Protein GI Number:
14250557
Purification:
APH1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
Q96BI3
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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