BANF1 Antibody

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ProSci
Product Code: PSI-4019
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4019-0.02mg0.02mg£150.00
Quantity:
PSI-4019-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: BANF1, 4017 (2 μg/mL), BANF1, 4019 (2 μg/mL) and beta-actin (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
2 / 5
<strong>Figure 2 Western Blot Validation in HeLa Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: BANF1 4019 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: BANF1, 4019 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Western Blot Validation in Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: BANF1, 4019 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 5 Immunofluorescence Validation of BANF1 in Human Kideny Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney tissue labeling BANF1 with 4019 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: BANF1, 4017 (2 μg/mL), BANF1, 4019 (2 μg/mL) and beta-actin (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in HeLa Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: BANF1 4019 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: BANF1, 4019 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: BANF1, 4019 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunofluorescence Validation of BANF1 in Human Kideny Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney tissue labeling BANF1 with 4019 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Documents

Further Information

Additional Names:
BANF1 Antibody: BAF, NGPS, BCRP1, D14S1460, BAF, BCRG1, Barrier-to-autointegration factor, Breakpoint cluster region protein 1
Application Note:
WB: 1-2 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
BANF1 Antibody: Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BAF also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BAF will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BAF is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA.
Background References:
  • Furukawa. J. Cell Sci. 1999; 112:2485-92.
  • Cai et al. EMBO J. 2001; 20:4399-407.
  • Chen and Engelman. Proc. Natl. Acad. Sci. USA 1998; 95:15270-4.
Buffer:
BANF1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
Anti-BANF1 antibody (4019) was raised against a peptide corresponding to 19 amino acids near the amino terminus of human BANF1.

The immunogen is located within the first 50 amino acids of BANF1.
ISOFORMS:
Human BANF1 has one isoform (89aa, 10kD). Mouse BANF1 has one isoform (89aa, 10kD) and Rat BANF1 also has one isoform (89aa, 10kD). 4019 can detect human, mouse and rat.
NCBI Gene ID #:
8815
NCBI Official Name:
barrier to autointegration factor 1
NCBI Official Symbol:
BANF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 10kD

Observed: 12 kD

Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE.
Protein Accession #:
AAH05942
Protein GI Number:
13543577
Purification:
BANF1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease
Swissprot #:
O75531
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar BANF1 expression profile in human and mouse cell lines detected by two independent anti-BANF1 antibodies that recognize different epitopes, 4017 against C-terminus domain and 4019 against N-terminus domain. BANF1 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

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