MDA5 Antibody
| Code | Size | Price |
|---|
| PSI-4037-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4037-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
MDA5 Antibody: Hlcd, MDA5, MDA-5, RLR-2, IDDM19, RH116, Interferon-induced helicase C domain-containing protein 1, Clinically amyopathic dermatomyositis autoantigen 140 kDa, CADM-140 autoantigen
Application Note:
MDA5 antibody can be used for detection of MDA5 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
MDA5 Antibody: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-κB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.
Background References:
- Akira S, Uematsu S, and Takeuchi O. Pathogen recognition and innate immunity. Cell 2006; 124:783-801.
- Hiscott J, Nguyen T-LA, Arguello M, et al. Manipulation of the nuclear factor-kappaB pathway and the innate immune response by viruses. Oncogene 2006; 25:6844-67.
- Kang D, Gopalrishnan RV, Lin L, et al. Expression analysis and genomic characterization of human melanoma differentiation associated gene-5, mda-5: a novel type I interferon-responsive apoptosis-inducing gene. Oncogene 2004; 23:1789-800.
- Andrejeva J, Childs KS, Young DF, et al. The V proteins of the paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter. Proc. Natl. Acad. Sci. USA 2004; 101:17264-9.
Buffer:
MDA5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MDA5 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MDA5.
The immunogen is located within the last 50 amino acids of MDA5.
The immunogen is located within the last 50 amino acids of MDA5.
NCBI Gene ID #:
64135
NCBI Official Name:
interferon induced with helicase C domain 1
NCBI Official Symbol:
IFIH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 113 kDa
Observed: 115 kDa
Observed: 115 kDa
Protein Accession #:
NP_071451
Protein GI Number:
27886568
Purification:
MDA5 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
Q9BYX4
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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