ProSci

TIGAR Antibody

Product Code:
 
PSI-4049
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 2
Western blot analysis of TIGAR in EL4 cell lysate with TIGAR antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
2 / 2
Immunohistochemistry of TIGAR in human brain tissue with TIGAR antibody at 2.5 μg/mL.
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Western blot analysis of TIGAR in EL4 cell lysate with TIGAR antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunohistochemistry of TIGAR in human brain tissue with TIGAR antibody at 2.5 μg/mL.

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CodeSizePrice
PSI-4049-0.02mg0.02mg£150.00
Quantity:
PSI-4049-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
TIGAR Antibody: FR2BP, TIGAR, C12orf5, Fructose-2, 6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator
Application Note:
TIGAR antibody can be used for detection of TIGAR by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
TIGAR Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death; loss of its functions contributes to the development of most cancers. Recent studies have demonstrated the ability of p53 to regulate the expression of several proteins involved in glycolysis and oxidative phosphorylation, such as TIGAR, SCO2, and phosphoglycerate mutase. TIGAR is a recently discovered protein that functions to regulate glycolysis and protect cells against oxidative stress. TIGAR is similar in structure to proteins in the phosphoglycerate mutase family, most notably 6-phosphofructo-2-kinase, suggesting TIGAR may function as a fructose bisphosphatase. Expression of TIGAR in transfected cells correlated with an inhibition of glycolysis and decreased levels of reactive oxygen species and p53-induced apoptosis, indicating that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses.
Background References:
  • Guimaraes DP and Hainaut P. TP53: a key gene in human cancer. Biochimie2002; 84:83-93.
  • Corcoran CA, Huang Y, and Sheikh MS. The regulation of energy generating pathways by p53. Cancer Biol. Ther.2006; 5:1610-3.
  • Bensaad K, Tsuruta A, Selak MA, et al. TIGAR, a p53-inducible regulator of glycolysis and apoptosis. Cell2006; 126:107-20.
Buffer:
TIGAR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
TIGAR antibody was raised against a 18 amino acid synthetic peptide from near the center of human TIGAR.

The immunogen is located within amino acids 60 - 110 of TIGAR.
NCBI Gene ID #:
57103
NCBI Official Name:
chromosome 12 open reading frame 5
NCBI Official Symbol:
C12orf5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_065108
Protein GI Number:
9966849
Purification:
TIGAR Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
Q9NQ88
User NOte:
Optimal dilutions for each application to be determined by the researcher.