ProSci

VISA Antibody

Product Code:
 
PSI-4055
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of VISA in rat brain tissue lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
2 / 3
Immunohistochemistry of VISA in mouse brain tissue with VISA antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of VISA in Mouse Brain cells with VISA antibody at 20 μg/mL.
❮❯

Western blot analysis of VISA in rat brain tissue lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunohistochemistry of VISA in mouse brain tissue with VISA antibody at 2.5 μg/mL.
Immunofluorescence of VISA in Mouse Brain cells with VISA antibody at 20 μg/mL.

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CodeSizePrice
PSI-4055-0.02mg0.02mg£150.00
Quantity:
PSI-4055-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
VISA Antibody: IPS1, VISA, IPS-1, CARDIF, IPS1, KIAA1271, Mitochondrial antiviral-signaling protein, CARD adapter inducing interferon beta, MAVS
Application Note:
VISA antibody can be used for detection of VISA by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
VISA Antibody: Two distinct signaling pathways activate the host innate immunity against viral infection. One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response. VISA is a mitochondrial membrane protein that was identified as a critical component in the IFN-b signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF-κB. VISA is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6. VISA also interacts with the IKKα, IKKβ and IKKε kinases through its C-terminal region. Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system. At least three isoforms of VISA are known to exist.
Background References:
  • Seth RB, Sun L, and Chen ZJ. Antiviral innate immunity pathways. Cell Res.2006; 16:141-7.
  • Xu LG, Wang YY, Han KJ, et al. VISA is an adapter protein required for virus-triggered IFN-beta signaling. Mol. Cell2005; 19:727-40.
  • Meylan E, Curran J, Hofman K, et al. Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus. Nature2005; 1167-72.
Buffer:
VISA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
VISA antibody was raised against a 17 amino acid synthetic peptide from near the center of human VISA.

The immunogen is located within amino acids 130 - 180 of VISA.
NCBI Gene ID #:
57506
NCBI Official Name:
mitochondrial antiviral signaling protein
NCBI Official Symbol:
MAVS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_065797
Protein GI Number:
83776598
Purification:
VISA Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
Q7Z434
User NOte:
Optimal dilutions for each application to be determined by the researcher.