PD-1 Antibody

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ProSci
Product Code: PSI-4067
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4067-0.02mg0.02mg£150.00
Quantity:
PSI-4067-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 4
Western blot analysis of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
2 / 4
Immunohistochemistry of PD-1 in human brain tissue with PD-1 antibody at 5 μg/mL.
3 / 4
Immunofluorescence of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 20 μg/ml.<br><br>Green: PD-1 Antibody (4067) <br> Blue: DAPI staining
4 / 4
Immunocytochemistry of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 10 μg/ml.
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Western blot analysis of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Immunohistochemistry of PD-1 in human brain tissue with PD-1 antibody at 5 μg/mL.
Immunofluorescence of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 20 μg/ml.<br><br>Green: PD-1 Antibody (4067) <br> Blue: DAPI staining
Immunocytochemistry of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 10 μg/ml.

Documents

Further Information

Additional Names:
PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1
Application Note:
PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry at 10 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.
Background References:
  • Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
  • Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
  • Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8.
  • Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity1999; 11:141-51.
Buffer:
PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PD-1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human PD-1.

The immunogen is located within amino acids 120 - 170 of PD-1.
NCBI Gene ID #:
5133
NCBI Official Name:
programmed cell death 1
NCBI Official Symbol:
PDCD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 32 kDa

Observed: 43 kDa
Protein Accession #:
Q15116
Protein GI Number:
145559515
Purification:
PD-1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
Q15116
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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