BRSK1 Antibody

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ProSci
Product Code: PSI-4081
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4081-0.02mg0.02mg£150.00
Quantity:
PSI-4081-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of BRSK1 in human brain tissue lysate with BRSK1 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of BRSK1 in human brain tissue with BRSK1 antibody at 2.5 μg/mL.
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Immunofluorescence of BRSK1 in Human Brain cells with BRSK1 antibody at 20 μg/mL.
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Western blot analysis of BRSK1 in human brain tissue lysate with BRSK1 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of BRSK1 in human brain tissue with BRSK1 antibody at 2.5 μg/mL.
Immunofluorescence of BRSK1 in Human Brain cells with BRSK1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
BRSK1 Antibody: hSAD1, KIAA1811, SAD1, SADB, Brain-selective kinase 1, SAD1 homolog
Application Note:
BRSK1 antibody can be used for detection of BRSK1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
BRSK1 Antibody: BRSK1 was initially identified as a mammalian homolog to the fission yeast S. pombe Cdr2, a mitosis-regulatory kinase and also shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK1 is unbiquitously expressed, with highest levels of expression in the brain and testes. Similar to its yeast homolog, BRSK1 is thought to be involved in stress-induced cell cycle arrest. Overexpression of this protein leads to the G2/M arrest in HeLa S2 cells and UV-induced G2/M arrest could be partially abrogated by reduced expression of BRSK1 through the use of siRNA, indicating its role in DNA damage checkpoint function. More recently, it has been shown that both BRSK1 and the related protein BRSK2 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. At least two isoforms of BRSK1 are known to exist.
Background References:
  • Lu R, Niida H, and Nakanishi M. Human SAD1 kinase is involved in UV-induced DNA damage checkpoint function. J. Biol. Chem.2004; 279:31164-70.
  • Kishi M, Pan YA, Crump J, et al. Mammalian SAD kinases are required for neuronal polarization. Science2005; 307:929-32.
Buffer:
BRSK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
BRSK1 antibody was raised against a 28 amino acid synthetic peptide from near the carboxy terminus of human BRSK1.

The immunogen is located within amino acids 720 - 770 of BRSK1.
NCBI Gene ID #:
84446
NCBI Official Name:
BR serine/threonine kinase 1
NCBI Official Symbol:
BRSK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q8TDC3
Protein GI Number:
34395684
Purification:
BRSK1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
Q8TDC3
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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