p53AIP1 Antibody

Product Code: PSI-2449

Supplier: ProSci

Code	Size	Price

PSI-2449-0.02mg

0.02mg

£150.00

Quantity:

PSI-2449-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Western Blot (WB)

Images

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Western blot analysis of p53AIP1 in HL60 cell lysate with p53AIP1 antibody at (A) 4 and (B) 8 μg/mL.

2 / 3

Immunocytochemistry of p53AIP1 in HL60 cells with p53AIP1 antibody at 10 μg/mL.

3 / 3

Immunofluorescence of p53AIP1 in HL60 cells with p53AIP1 antibody at 20 μg/mL.

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Documents

Further Information

Additional Names:

p53AIP1 Antibody: P53AIP1, p53-regulated apoptosis-inducing protein 1, p53AIP1

Application Note:

p53AIP1 antibody can be used for detection of p53AIP1 by Western blot at 4 - 8 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Background:

p53AIP1 Antibody: The p53 tumor-suppressor protein can induce apoptosis through transcriptional activation of several genes. One such protein p53AIP was initially identified through direct cloning of p53 binding sequences from human genomic DNA. Its expression is inducible by p53 following p53 phosphorylation on Ser-46, and ectopic expression of p53AIP leads to apoptotic cell death. Both the phosphorylation of p53 and the induction of p53AIP were blocked by inhibiting the expression of p53DINP1 by the introduction of antisense oligonucleotides to p53DINP1, suggesting that the apoptosis associated with p53AIP expression is regulated by p53DINP1. Finally, as adenovirus-mediated introduction of p53AIP has been shown to suppress tumor growth in vivo, it has been suggested that p53AIP gene transfer may become a useful strategy for the treatment of p53-resistant cancers. Three isoforms of p53AIP are known to exist; this antibody will detect all three.

Background References:

Kern SE, Pietenpol JA, Thiagalingam S, Seymour A, et al. Oncogenic forms of p53 inhibit p53-regulated gene expression. Science1991; 252:1708-11.
Oda K, Arakawa H, Tanaka T, et al. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell2000; 102:849-52.
Okamura S, Arakawa H, Tanaka T, et al. p53DINP1, a p53-inducible gene, regulates p53-dependent apoptosis. Mol. Cell2001; 8:85-94.
Yoshida K, Monden M, Nakamura Y, et al. Adenovirus-mediated p53AIP1 gene transfer as a new strategy for treatment of p53-resistant tumors. Cancer Sci.2004; 95:91-7.

Buffer:

p53AIP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/ml

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

p53AIP1 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human p53AIP1.

The immunogen is located within the first 50 amino acids of p53AIP1.

NCBI Gene ID #:

63970

NCBI Official Name:

tumor protein p53 regulated apoptosis inducing protein 1

NCBI Official Symbol:

TP53AIP1

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Protein Accession #:

BAB16421

Protein GI Number:

10798768

Purification:

p53AIP1 Antibody is affinity chromatography purified via peptide column.

Research Area:

Apoptosis,Cancer

Swissprot #:

Q9HCN2

User NOte:

Optimal dilutions for each application to be determined by the researcher.

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