DARC Antibody

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ProSci
Product Code: PSI-4069
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4069-0.02mg0.02mg£150.00
Quantity:
PSI-4069-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of DARC in human cerebellum tissue lysate with DARC antibody at 1 μg/mL.
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Immunohistochemistry of DARC in mouse brain tissue with DARC antibody at 2.5 μg/mL.
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Western blot analysis of DARC in human cerebellum tissue lysate with DARC antibody at 1 μg/mL.
Immunohistochemistry of DARC in mouse brain tissue with DARC antibody at 2.5 μg/mL.

Documents

Further Information

Additional Names:
DARC Antibody: FY, Dfy, GPD, GpFy, ACKR1, CCBP1, CD234, WBCQ1, FY, Atypical chemokine receptor 1
Application Note:
DARC antibody can be used for detection of DARC by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
DARC Antibody: DARC, also known as the Duffy antigen/chemokine receptor, is a seven-transmembrane protein homologous to the classical chemokine G-protein coupled receptors (GPCRs) with the exception of the motif required for G protein coupling. DARC can bind with high affinity several chemokines without transducing any signal, suggesting it may modulate the signals normally induced by these chemokines. Recently, DARC was found to interact with KAI1, a four transmembrane protein recently identified as a tumor metastasis suppressor protein. It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least three isoforms of DARC are known to exist.
Background References:
  • Chaudhuri A, Polyakova J, Zbrzezna V, et al. Cloning of glycoprotein D cDNA, which encodes the major subunit of the Duffy blood group system and the receptor for the Plasmodium vivax malaria parasite. Proc. Natl. Acad. Sci. USA1993; 90:10793-7.
  • Gardner L, Patterson AM, Ashton BA, et al. The human Duffy antigen binds selected inflammatory but not homeostatic chemokines. Biochem. Biophys. Res. Commun.2004; 321:306-12.
  • Gil ML, Vita N, Lebel-Binay S, et al. A member of the tetra spans transmembrane protein superfamily is recognized by a monoclonal antibody raised against an HLA class I-deficient, lymphokine-activated killer-susceptible, B lymphocyte line. Cloning and functional studies. J. Immunol.1992; 2826-33.
Buffer:
DARC Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DARC antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human DARC.

The immunogen is located within the last 50 amino acids of DARC.
NCBI Gene ID #:
2532
NCBI Official Name:
Duffy blood group, chemokine receptor
NCBI Official Symbol:
DARC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q16570
Protein GI Number:
67476970
Purification:
DARC Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q16570
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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