MCG10 Antibody

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ProSci
Product Code: PSI-2479
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2479-0.02mg0.02mg£150.00
Quantity:
PSI-2479-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of MCG10 in HeLa cell lysate with MCG10 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
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Immunocytochemistry of MCG10 in HeLa cells with MCG10 antibody at 1 μg/mL.
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Immunofluorescence of MCG10 in Hela cells with MCG10 antibody at 20 μg/mL.
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Western blot analysis of MCG10 in HeLa cell lysate with MCG10 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunocytochemistry of MCG10 in HeLa cells with MCG10 antibody at 1 μg/mL.
Immunofluorescence of MCG10 in Hela cells with MCG10 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
MCG10 Antibody: CBP, LIP4, MCG10Alpha-CP4
Application Note:
MCG10 antibody can be used for detection of MCG10 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
MCG10 Antibody: MCG10 is a member of the KH-domain RNA-binding protein family. Proteins of this family also referred to as alpha-CPs, bind to RNA with specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. MCG10 is induced by p53 and DNA damage via two p53-responsive promoter elements. Its overexpression in transfected cells results in the suppression of cell proliferation by inducing apoptosis and cell cycle arrest in G2-M. Decreased expression of one isoform of MCG10 has been observed in many forms of lung cancer, leading to speculation that MCG10 can act as a tumor suppressor. Multiple alternatively spliced isoforms of MCG10 have been identified.
Background References:
  • Makeyev AV and Liebhaber SA. The poly(C)-binding proteins: a multiplicity of functions and a search for mechanisms. RNA2002; 8:265-78.
  • Zhu J and Chen X. MCG10, a novel p53 target gene that encodes a KH domain RNA-binding protein, is capable of inducing apoptosis and cell cycle arrest in G2-M. Mol. Cell Biol.2000; 20:5602-5618.
  • Pio R, Zudaire I, Pino I, et al. a-CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its alternative splice variant aCP-4a, is underexpressed in lung cancer. Cancer Res.2004; 64:4171-9.
Buffer:
MCG10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (100%), Bovine: (100%)
Immunogen:
MCG10 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MCG10.

The immunogen is located within the last 50 amino acids of MCG10.
NCBI Gene ID #:
57060
NCBI Official Name:
poly(rC) binding protein 4
NCBI Official Symbol:
PCBP4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
EAW65166
Protein GI Number:
119585570
Purification:
MCG10 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
P57723
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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