NPAS3 Antibody
| Code | Size | Price |
|---|
| PSI-4107-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4107-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
NPAS3 Antibody: MOP6, PASD6, bHLHe12, BHLHE12, MOP6, Neuronal PAS domain-containing protein 3, Basic-helix-loop-helix-PAS protein MOP6, Neuronal PAS3
Application Note:
NPAS3 antibody can be used for detection of NPAS3 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
NPAS3 Antibody: Neuronal PAS domain protein 3 (NPAS3) is a brain-enriched basic helix-loop-helix PAS domain transcription factor and is broadly expressed in the developing neuroepithelium and has recently found to be disrupted by genetic translocation in a family affected with schizophrenia. It was recently shown to be involved in the regulation of FGF signaling in the dentate gyrus by controlling the expression of the FGF receptor subtype 1 and in turn neurogenesis emanating from this region. NPAS3-null mice were growth-retarded and displayed brain defects that included reduced size of the anterior hippocampus, hypoplasia of the corpus callosum, and enlargement of the ventricles, as well as several behavioral abnormalities. Furthermore, these NPAS3-null mice also exhibited disruptions in several neurosignaling pathways involving glutamate, dopamine, and serotonin. These results demonstrate the essential role played by NPAS3 during structural and functional brain development. At least three isoforms of NPAS3 are known to exist.
Background References:
- Brunskill EW, Witte DP, Shreiner AB et al. Characterization of npas3, a novel basic helix-loop-helix PAS gene expressed in the developing mouse nervous system. Mech. Dev.1999; 88:237-41.
- Kamnasaran D, Muir WJ, Ferguson-Smith MA, et al. Disruption of the neuronal PAS3 gene in a family affected with schizophrenia. J. Med. Genet.40:325-32.
- Brunskill EW, Ehrman LA, Williams MT, et al. Abnormal neurodevelopment, neurosignaling and behaviour in Npas3-deficient mice. Euro. J. Neurosci.2005; 22:1265-76.
Buffer:
NPAS3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (84%), Chicken: (80%)
Immunogen:
NPAS3 antibody was raised against a 28 amino acid synthetic peptide from near the amino terminus of human NPAS3.
The immunogen is located within amino acids 40 - 90 of NPAS3.
The immunogen is located within amino acids 40 - 90 of NPAS3.
NCBI Gene ID #:
64067
NCBI Official Name:
neuronal PAS domain protein 3
NCBI Official Symbol:
NPAS3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q8IXF0
Protein GI Number:
38604888
Purification:
NPAS3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
Q8IXF0
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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