NogoA Antibody
| Code | Size | Price |
|---|
| PSI-4089-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4089-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
NogoA Antibody: ASY, NSP, NOGO, NOGOC, RTN-X, NOGO-A, NSP-CL, Nogo-B, Nogo-C, RTN4-A, RTN4-C, RTN4-B1, RTN4-B2, NI220/250, Nbla00271, Nbla10545, KIAA0886, My043, SP1507, Reticulon-4, Foocen, Nogo protein
Application Note:
NogoA antibody can be used for detection of NogoA by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
NogoA Antibody: NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS; blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.
Background References:
- Yan R, ShiQ, Hu X, et al. Reticulon proteins: emerging players in neurodegenerative diseases. Cell. Mol. Life Sci. 2006; 63:877-889.
- Chen MS, Huber AB, van der Haar ME, et al. Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1. Nature 2000; 403:434-9.
- Schweigreiter R and Bandtlow CE. Nogo in the injured spinal cord. J. Neurotrauma 2006; 3-4:384-96.
- Dupuis L, Gonzalez de Aguilar JL, di Scala F, et al. Nogo provides a molecular marker for diagnosis of amyloid lateral sclerosis. Neurobiol. Dis. 2002; 10:358-65.
Buffer:
NogoA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
NogoA antibody was raised against a 19 amino acid synthetic peptide from near the center of human NogoA.
The immunogen is located within amino acids 200 - 250 of NogoA.
The immunogen is located within amino acids 200 - 250 of NogoA.
NCBI Gene ID #:
57142
NCBI Official Name:
reticulon 4
NCBI Official Symbol:
RTN4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 131 kDa
Observed: ~180 kDa
Observed: ~180 kDa
Protein Accession #:
NP_065393
Protein GI Number:
24431935
Purification:
NogoA Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
SPECIFICITY:
At least five isoforms of Nogo are known to exist; this antibody is specific for NogoA and NogoE.
Swissprot #:
Q9NQC3
User NOte:
Optimal dilutions for each application to be determined by the researcher.
Related Products
| Product Name | Product Code | Supplier | NogoA Peptide | PSI-4089P | ProSci | Summary Details | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|