ORAI3 Antibody
| Code | Size | Price |
|---|
| PSI-4117-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4117-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ORAI3 Antibody: TMEM142C, TMEM142C, Protein orai-3, Transmembrane protein 142C
Application Note:
ORAI3 antibody can be used for detection of ORAI3 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2 μg/mL
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Background:
ORAI3 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter.
Background References:
- Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
- Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
- Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.
- Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90.
Buffer:
ORAI3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ORAI3 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human ORAI3.
The immunogen is located within the first 50 amino acids of ORAI3.
The immunogen is located within the first 50 amino acids of ORAI3.
NCBI Gene ID #:
93129
NCBI Official Name:
ORAI calcium release-activated calcium modulator 3
NCBI Official Symbol:
ORAI3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9BRQ5
Protein GI Number:
74732916
Purification:
ORAI3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology,Cancer
SPECIFICITY:
This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2.
Swissprot #:
Q9BRQ5
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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