STIM1 Antibody

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ProSci
Product Code: PSI-4119
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4119-0.02mg0.02mg£150.00
Quantity:
PSI-4119-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of STIM1 in mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2 μg/mL.
2 / 5
Immunohistochemistry of STIM1 in human spleen tissue with STIM1 antibody at 2.5 μg/mL.
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Immunohistochemistry of STIM1 in mouse spleen tissue with STIM1 antibody at 5 μg/ml.
4 / 5
Immunofluorescence of STIM1 in mouse spleen tissue with STIM1 antibody at 20 μg/mL.<br><br>Green: STIM1 Antibody (4119) <br> Blue: DAPI staining
5 / 5
Immunofluorescence of STIM1 in Hek293 cells with STIM1 antibody at 20 μg/mL.<br><br>Red: STIM1 Antibody (4119) <br> Blue: DAPI staining
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Western blot analysis of STIM1 in mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of STIM1 in human spleen tissue with STIM1 antibody at 2.5 μg/mL.
Immunohistochemistry of STIM1 in mouse spleen tissue with STIM1 antibody at 5 μg/ml.
Immunofluorescence of STIM1 in mouse spleen tissue with STIM1 antibody at 20 μg/mL.<br><br>Green: STIM1 Antibody (4119) <br> Blue: DAPI staining
Immunofluorescence of STIM1 in Hek293 cells with STIM1 antibody at 20 μg/mL.<br><br>Red: STIM1 Antibody (4119) <br> Blue: DAPI staining

Documents

Further Information

Additional Names:
STIM1 Antibody: GOK, TAM, IMD10, D11S4896E, GOK, Stromal interaction molecule 1
Application Note:
STIM1 antibody can be used for detection of STIM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
STIM1 Antibody: In T lymphocytes, the sole pathway for Ca2+ entry following antigen-receptor binding is through store-operated Ca2+-release-activated Ca2+ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca2+ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca2+ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca2+ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca2+ entry.
Background References:
  • Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated Ca2+ signaling in T cells. Trends Mol. Med. 2007; 13:103-7.
  • Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
  • Zhang SL, Yu Y, Roos J, et al. STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane. Nature 2005; 437:902-5.
  • Spassova MA, Soboloff J, He L-P, et al. STIM1 has a plasma membrane role in the activation of store-operated Ca2+ channels. Proc. Natl. Acad. Sci. USA 2006; 103:4040-5.
Buffer:
STIM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
STIM1 antibody was raised against a 24 amino acid synthetic peptide from near the carboxy terminus of human STIM1.

The immunogen is located within the last 50 amino acids of STIM1.
NCBI Gene ID #:
6786
NCBI Official Name:
stromal interaction molecule 1
NCBI Official Symbol:
STIM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 59, 75, 87 kDa

Observed: 88 kDa
Protein Accession #:
NP_003147
Protein GI Number:
21070997
Purification:
STIM1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology,Cancer
SPECIFICITY:
At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2.
Swissprot #:
Q13586
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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