ARF-BP1 Antibody

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ProSci
Product Code: PSI-4213
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4213-0.02mg0.02mg£150.00
Quantity:
PSI-4213-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 μg/mL.
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Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 μg/mL.
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Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 μg/mL.
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Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 μg/mL.
Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 μg/mL.
Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
ARF-BP1 Antibody: MULE, Ib772, LASU1, UREB1, HECTH9, URE-B1, ARF-BP1, HSPC272, KIAA0312, KIAA1578, E3 ubiquitin-protein ligase HUWE1, ARF-binding protein 1
Application Note:
ARF-BP1 antibody can be used for detection of ARF-BP1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ARF-BP1 Antibody: The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.
Background References:
  • Gallagher SJ, Kefford RF and Rizos H. The ARF tumour suppressor. Int. J. Biochem. Cell Biol.2006; 38:1637-41.
  • Pomerantz J, Schreiber-Agus N, Leigeois NJ, et al. The Ink4a tumor suppressor gene product, p19ARF interacts with MDM2 and neutralizes MDM2's inhibition of p53. Cell1998; 92:725-34.
  • Chen D, Kon N, Li M, et al. ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor. Cell2005; 121:1071-83.
  • Zhong Q, Gao W, Du F, et al. Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis. Cell2005; 121:1085-95.
Buffer:
ARF-BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ARF-BP1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human ARF-BP1.

The immunogen is located within amino acids 4250 - 4300 of ARF-BP1.
NCBI Gene ID #:
10075
NCBI Official Name:
HECT, UBA and WWE domain containing 1
NCBI Official Symbol:
HUWE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_113584
Protein GI Number:
61676188
Purification:
ARF-BP1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
Q7Z6Z7
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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