Presenilin1 Antibody
| Code | Size | Price |
|---|
| PSI-4203-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4203-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Presenilin1 Antibody: AD3, FAD, PS1, PS-1, S182, AD3, PSNL1, Presenilin-1, Protein S182
Application Note:
Presenilin1 antibody can be used for detection of presenilin1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Presenilin1 Antibody: Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimer's disease. In addition to PEN2, nicastrin and APH-1, Presenilin1 forms the gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Multiple isoforms of presenilin1 are known to exist. This antibody has no cross-reactivity to presenilin2.
Background References:
- Sherrington R, Rogaev EI, Liang Y, et al. Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease. Nature1995; 375:754-60.
- Weihofen A and Martoglio B. Intramembrane-cleaving proteases: controlled liberation of proteins and bioactive peptides. Trends Cell Biol.2003; 13:71-8.
- Periz G and Fortini ME. Functional reconstitution of g-secretase through coordinated expression of presenilin, nicastrin, aph-1, and pen-2. J. Neurosci. Res.2004; 77:309-22.
- Selkoe DJ. The cell biology of b-amyloid precursor protein and presenilin in Alzheimer's disease. Trends Cell Biol.1998; 8:447-53.
Buffer:
Presenilin1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (86%)
Immunogen:
Presenilin1 antibody was raised against a 23 amino acid synthetic peptide from near the carboxy terminus of human presenilin1.
The immunogen is located within amino acids 330 - 380 of Presenilin1.
The immunogen is located within amino acids 330 - 380 of Presenilin1.
NCBI Gene ID #:
5663
NCBI Official Name:
presenilin 1
NCBI Official Symbol:
PSEN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000012
Protein GI Number:
4506163
Purification:
Presenilin1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
P49768
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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