Bub1 Antibody
| Code | Size | Price |
|---|
| PSI-4229-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4229-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Bub1 Antibody: BUB1A, BUB1L, hBUB1, BUB1A
Application Note:
Bub1 antibody can be used for detection of bub1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
Bub1 Antibody: Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.
Background References:
- Pangilinan F, Li Q, Weaver T, et al. Mammalian BUB1 protein kinases: map positions and in vivo expression. Genomics1997; 46:379-88.
- Williams GL, Roberts TM and Gjoerup OV. Bub1: Escapades in a cellular world. Cell Cycle2007; 6:1699-704.
- Meraldi P and Sorger PK. A dual role for Bub1 in the spindle checkpoint and chromosome congression. EMBO J.2005; 24:1621-33.
- Tang Z, Shu H, Oncel D, et al. Phosphorylation of cdc20 by bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint. Mol. Cell2004; 16:387-97.
Buffer:
Bub1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Bub1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1.
The immunogen is located within amino acids 40 - 90 of Bub1.
The immunogen is located within amino acids 40 - 90 of Bub1.
NCBI Gene ID #:
699
NCBI Official Name:
budding uninhibited by benzimidazoles 1 homolog (yeast)
NCBI Official Symbol:
BUB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
O43683
Protein GI Number:
8134347
Purification:
Bub1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Signal Transduction,Cancer
Swissprot #:
O43683
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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