op18 Antibody

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ProSci
Product Code: PSI-4235
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4235-0.02mg0.02mg£150.00
Quantity:
PSI-4235-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 1 Western Blot Validation in Mouse EL4 Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: op18 4235 (A: 0.5 ug/mL, B: 1 μg /mL, C: 2 μg /mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: op18 4235 (2 μg/mL), op18 4237 (2 μg /mL), and GAPDH 3783 (0.02 μg /mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of cell lysate per lane. Antibodies: op18 4235 (1 μg /mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Western Blot Validation in Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: op18 4235 (1μg /mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 5 Immunohistochemistry Validation of op18 in Human Brain Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-op18 antibody (4235) at 2.5 μg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 1 Western Blot Validation in Mouse EL4 Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: op18 4235 (A: 0.5 ug/mL, B: 1 μg /mL, C: 2 μg /mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: op18 4235 (2 μg/mL), op18 4237 (2 μg /mL), and GAPDH 3783 (0.02 μg /mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of cell lysate per lane. Antibodies: op18 4235 (1 μg /mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: op18 4235 (1μg /mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunohistochemistry Validation of op18 in Human Brain Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-op18 antibody (4235) at 2.5 μg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Documents

Further Information

Additional Names:
op18 Antibody: Lag, SMN, OP18, PP17, PP19, PR22, LAP18, C1orf215, Stathmin, Leukemia-associated phosphoprotein p18, Op18
Application Note:
WB: 0.5-2 μg /mL; IHC: 2.5 μg /mL; IF: 20 μg /mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
op18 Antibody: Op18 belongs to the stathmin family of genes and encodes a ubiquitous cytosolic phosphoprotein that may function as an intracellular relay integrating several signaling pathways such as those involved in cell proliferation and differentiation. Op18 has also been shown to be involved in the regulation of the microtubule filament system by destabilizing microtubules, thereby preventing assembly and promoting the disassembly of microtubules. More recently, op18 has been implicated as a potential target of the ASK1-p38 MAP kinase cascade, suggesting that the ASK1-p38 cascade may regulate microtubule dynamics through op18. Op18 is highly expressed in a wide variety of human malignancies, including leukemia, prostate cancer, ovarian carcinoma, and breast carcinoma, suggesting that op18 may be an ideal target for anti-cancer therapeutics.
Background References:
  • Curmi et al. Cell Structure and Function 1999; 24:345-57.
  • Belmont et al. Cell 1996; 84:623-31.
  • Mizumura et al. J. Cell Physiol. 2006; 206:363-70.
  • Mistry et al. Mount Sinai J. Med. 2002; 69:299-304.
Buffer:
op18 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (100%), Chicken: (95%)
Immunogen:
Anti-op18 antibody (4235) was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human op18.

The immunogen is located within the last 50 amino acids of op18.
ISOFORMS:
Human op18 has 2 isoforms, including isoform 1 (149aa, 17kD) and isoform 2 (174aa, 20kD). Mouse op18 has one isoform (149aa, 17kD) and rat op18 also has one isoform (149aa, 17kD). 4235 can detect only human isoform 1and it can also detect mouse and rat op18.
NCBI Gene ID #:
3925
NCBI Official Name:
STMN1
NCBI Official Symbol:
STMN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 17kD

Observed: 17 kD
Protein Accession #:
AAH82228
Protein GI Number:
51895905
Purification:
op18 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
P16949
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar op18 expression profile in human, mouse and rat cell lines detected by two independent anti-op18 antibodies that recognize different epitopes, 4235 against C-terminus domain and 4237 against N-terminus domain. op18 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

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