Bub3 Antibody
| Code | Size | Price |
|---|
| PSI-4227-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4227-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Bub3 Antibody: BUB3L, hBUB3, Mitotic checkpoint protein BUB3
Application Note:
Bub3 antibody can be used for detection of bub3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Bub3 Antibody: The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.
Background References:
- Kalitsis P, Earle E, Fowler KJ, et al. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis. Genes Dev.2000; 14:2277-82.
- Taylor SS, Ha E and McKeon F. The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase. J. Cell Biol.1998; 142:1-11.
- Warren CD, Brady DM, Johnston RC, et al. Distinct chromosome segregation roles for spindle checkpoint proteins. Mol. Biol. Cell2002; 13: 3029-41.
- Roberts BT, Farr KA and Hoyt MA. The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. Mol. Cell. Biol.1994; 14:8282-91.
Buffer:
Bub3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
Bub3 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human bub3.
The immunogen is located within the last 50 amino acids of Bub3.
The immunogen is located within the last 50 amino acids of Bub3.
NCBI Gene ID #:
9184
NCBI Official Name:
budding uninhibited by benzimidazoles 3 homolog (yeast)
NCBI Official Symbol:
BUB3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
O43684
Protein GI Number:
7387554
Purification:
Bub3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Signal Transduction
Swissprot #:
O43684
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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