NK3R Antibody
| Code | Size | Price |
|---|
| PSI-4255-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4255-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
NK3R Antibody: NKR, HH11, NK3R, NK-3R, TAC3RL, TAC3R, Neuromedin-K receptor, NK-3 receptor, NKR
Application Note:
NK3R antibody can be used for detection of NK3R by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
NK3R Antibody: The tachykinins are a family of small peptides that include the neurotransmitters substance P, neurokinin A, and neurokinin B, which can act on three related but distinct seven transmembrane G-proteins coupled receptors, albeit at different concentrations. The NK-3 receptor (NK3R) has greatest affinity for neurokinin B and is highly expressed in the supraoptic and paraventricular nuclei. Following binding of its ligand, NK3R activates a phosphatidylinositol-calcium second messenger system. It is likely these signals lead to the release of vasopressin and oxytocin into the circulation. NK3R may be involved in learning and memory as mice lacking this gene expressed cognitive deficits compared to normal mice. Although it has been suggested that NK3R plays a role in the regulation of vagal afferent relay neurons, it is likely that these receptors are activated by substance P or neurokinin A, as the airway nerves do not express neurokinin B.
Background References:
- Maggi CA. The mammalian tachykinin receptors. Gen. Pharmacol. 1995; 26:911-44.
- Ding Y-Q, Shi J, Su L-Y, et al. Receptor (NK3)-containing neurons in the paraventricular and supraoptic nuclei of the rat hypothalamus synthesize vasopressin and express fos following intravenous injection of hypotonic saline. Neurosci. 1999; 91:1077-85.
- Nakajima Y, Tsuchida K, Negishi M, et al. Direct linkage of three tachykinin receptors to stimulation of both phosphatidylinositol hydrolysis and cyclic AMP cascades in transfected Chinese hamster ovary cells. J. Biol. Chem. 1992; 267:2437-42.
- Haley GE and Flynn FW. Tachykinin NK3 receptor contribution to systemic release of vasopressin and oxytocin in response to osmotic and hypotensive challenge. Am. J. Regul. Integr. Comp. Physiol. 2007; 293:R931-7.
Buffer:
NK3R Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (94%), Rabbit: (94%), Guinea pig: (83%)
Immunogen:
NK3R antibody was raised against a 18 amino acid synthetic peptide from near the center of human NK3R.
The immunogen is located within amino acids 370 - 420 of NK3R.
The immunogen is located within amino acids 370 - 420 of NK3R.
NCBI Gene ID #:
6870
NCBI Official Name:
tachykinin receptor 3
NCBI Official Symbol:
TACR3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 51 kDa
Observed: 51 kDa
Observed: 51 kDa
Protein Accession #:
P29371
Protein GI Number:
128364
Purification:
NK3R Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
SPECIFICITY:
NK3R antibody does not recognize NK1R or NK2R.
Swissprot #:
P29371
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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