MICA Antibody
| Code | Size | Price |
|---|
| PSI-4261-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4261-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
MICA Antibody: PERB11.1, PERB11.1, MHC class I polypeptide-related sequence A, MIC-A
Application Note:
MICA antibody can be used for detection of MICA by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
MICA Antibody: Major histocompatibility complex (MHC) class I proteins are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. A related family, termed the MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. Both MICA and MICB mRNA are widely expressed in normal tissues, with MICA being present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.
Background References:
- Rudolph MG, Stanfield RL and Wilson IA. How TCRs bind MHCs, peptides, and coreceptors. Annu. Rev. Immunol. 2006; 24:419-66.
- Bahram S, Bresnahan M, Geraghty DE, et al. A second lineage of mammalian major histocompatibility complex I genes. Proc. Natl. Acad. Sci. USA 1994; 91:6259-63.
- Bauer S, Groh V, Wu J, et al. Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science 1999; 285:727-9.
- Maccalli C, Pende D, Castelli C, et al. NKG2D engagement of colorectal cancer-specific T cells strengthens TCR-mediated antigen stimulation and elicits TCR independent anti-tumor activity. Eur. J. Immunol. 2003; 33:2033-43.
Buffer:
MICA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MICA antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MICA.
The immunogen is located within the last 50 amino acids of MICA.
The immunogen is located within the last 50 amino acids of MICA.
NCBI Gene ID #:
4276
NCBI Official Name:
MHC class I polypeptide-related sequence A
NCBI Official Symbol:
MICA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 42 kDa
Observed: 46 kDa
Observed: 46 kDa
Protein Accession #:
NP_000238
Protein GI Number:
4557751
Purification:
MICA Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
Swissprot #:
Q29983
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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