Carabin Antibody

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ProSci
Product Code: PSI-4269
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4269-0.02mg0.02mg£150.00
Quantity:
PSI-4269-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of Carabin in human spleen tissue lysate with Carabin antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of Carabin in human spleen tissue with Carabin antibody at 2.5 μg/mL.
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Western blot analysis of Carabin in human spleen tissue lysate with Carabin antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of Carabin in human spleen tissue with Carabin antibody at 2.5 μg/mL.

Documents

Further Information

Additional Names:
Carabin Antibody: EPI64C, CARABIN, Carabin, TBC1 domain family member 10C
Application Note:
Carabin antibody can be used for detection of Carabin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
Carabin Antibody: Antigen binding by the T-cell receptor (TCR) is one of the critical first steps in the immune response, triggering a cascade of signaling pathways that ultimately lead to T-cell activation. Screening a yeast two-hybrid screen of a human T-cell cDNA library with calcineurin, a protein phosphatase involved in multiple signaling pathways including T-cell activation, resulted in the identification of Carabin, a member of the TBC1 domain family of proteins, as a calcineurin-binding protein. Unlike other members of the TBC1 domain protein family which are thought to have a role in regulating cell growth and differentiation, further experiments demonstrated that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway as well as an inhibitor of the Ras signaling pathway, suggesting that Carabin may also mediate crosstalk between calcineurin and Ras. Carabin antibody does not recognize TBC1D10A or TBC1D10B. Carabin is known to exist in multiple isoforms.
Background References:
  • Weil R and Israel A. Deciphering the pathway from the TCR to NF-kappaB. Cell Death Differ.2006; 13:826-33.
  • Im SH and Rao A. Activation and deactivation of gene expression Ca2+/calcineurin-NFAT-mediated signaling. Mol. Cells2004; 18:1-9.
  • Pan F, Sun L, Kardian DB, et al. Feedback inhibition of calcineurin and ras by a dual inhibitory protein carabin. Nature2007; 445:433-6.
  • White RA, Pasztor LM, Richardson PM, et al. The gene encoding TBC1D1 with homology to the tre-2/USP6 oncogene, Bub2, and cdc16 maps to mouse chromosome 5 and human chromosome 4. Cytogenet. Cell Genet.2000; 89:272-5.
Buffer:
Carabin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Carabin antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Carabin.

The immunogen is located within amino acids 100 - 150 of Carabin.
NCBI Gene ID #:
374403
NCBI Official Name:
TBC1 domain family, member 10C
NCBI Official Symbol:
TBC1D10C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_940919
Protein GI Number:
38348348
Purification:
Carabin Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
Swissprot #:
Q8IV04
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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