IL-33 Antibody

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ProSci
Product Code: PSI-4271
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4271-0.02mg0.02mg£150.00
Quantity:
PSI-4271-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of IL-33 in human lymph node tissue lysate with IL-33 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunofluorescence of IL-33 in human lymph node tissue with IL-33 antibody at 20 μg/mL.
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Immunofluorescence of IL-33 in Human Lymph Node cells with IL-33 antibody at 20 μg/mL.
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Western blot analysis of IL-33 in human lymph node tissue lysate with IL-33 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of IL-33 in human lymph node tissue with IL-33 antibody at 20 μg/mL.
Immunofluorescence of IL-33 in Human Lymph Node cells with IL-33 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
IL-33 Antibody: DVS27, IL1F11, NF-HEV, NFEHEV, C9orf26, NFHEV, Interleukin-33, Interleukin-1 family member 11, IL-33
Application Note:
IL-33 antibody can be used for the detection of IL-33 by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
IL-33 Antibody: Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1α/beta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-κB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties.
Background References:
  • Schmitz J, Owyang A, Oldham E, et al. IL-33, and interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity2005; 23:479-90.
  • Dinarello CA. Interleukin-18, a proinflammatory cytokine. Eur. Cytokine Netw.2000; 11:483-6.
  • Brint EK, Xu D, Liu H, et al. ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance. Nat. Immunol.2004; 5:373-9.
  • Chackerian AA, Oldham ER, Murphy EE, et al. IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex. J. Immunol.2007; 179:2551-5.
Buffer:
IL-33 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (72%)
Immunogen:
IL-33 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human IL-33.

The immunogen is located within amino acids 50 - 100 of IL-33.
NCBI Gene ID #:
90865
NCBI Official Name:
interleukin 33
NCBI Official Symbol:
IL33
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_254274
Protein GI Number:
15559209
Purification:
IL-33 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
Swissprot #:
O95760
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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