ADAP Antibody
| Code | Size | Price |
|---|
| PSI-4279-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4279-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ADAP Antibody: ADAP, PRO0823, SLAP-130, SLAP130, FYN-binding protein, Adhesion and degranulation promoting adaptor protein, ADAP
Application Note:
ADAP antibody can be used for detection of ADAP by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ADAP Antibody: The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-κB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.
Background References:
- Griffiths EK, Krawczyk C, Kong YY, et al. Positive regulation of T cell activation and integrin adhesion by the adapter Fyb/Slap. Science2001; 293:2260-3.
- Rawlings DJ, Sommer K, and Moreno-Garcia ME. The CARMA1 signalosome links the signalling machinery of adaptive and innate immunity in lymphocytes. Nat. Rev. Immunol.2006; 6:799-812.
- Medeiros RB, Burbach BJ, Mueller KL, et al. Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1. Science2007; 316:754-8.
- Mueller KL, Thomas MS, Burbach BJ, et al. Adhesion and degranulation-promoting adapter protein (ADAP) positively regulates T cell sensitivity to antigen and T cell survival. J. Immunol.2007; 179:3559-69.
Buffer:
ADAP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ADAP antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ADAP.
The immunogen is located within amino acids 60 - 110 of ADAP.
The immunogen is located within amino acids 60 - 110 of ADAP.
NCBI Gene ID #:
2533
NCBI Official Name:
FYN binding protein (FYB-120/130)
NCBI Official Symbol:
FYB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001456
Protein GI Number:
42476118
Purification:
ADAP Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
Swissprot #:
O15117
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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