ORAI1 Antibody
| Code | Size | Price |
|---|
| PSI-4281-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4281-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ORAI1 Antibody: IMD9, ORAT1, CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1
Application Note:
ORAI1 antibody can be used for detection of ORAI1 by Western blot at 0.5 - 2 μg/mL. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ORAI1 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor.
Background References:
- Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol.2001; 19:497-521.
- Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature2006; 441:179-85.
- Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta.2006; 1763:1161-8.
Buffer:
ORAI1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (93%), Chicken: (100%)
Immunogen:
ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.
The immunogen is located within the last 50 amino acids of ORAI1.
The immunogen is located within the last 50 amino acids of ORAI1.
NCBI Gene ID #:
84876
NCBI Official Name:
ORAI calcium release-activated calcium modulator 1
NCBI Official Symbol:
ORAI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q96D31
Protein GI Number:
97180269
Purification:
ORAI1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
SPECIFICITY:
This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
Swissprot #:
Q96D31
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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