STEAP1 Antibody

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ProSci
Product Code: PSI-4303
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4303-0.02mg0.02mg£150.00
Quantity:
PSI-4303-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of STEAP1 in human spleen tissue lysate with STEAP1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of STEAP1 in human spleen tissue with STEAP1 antibody at 2.5 μg/mL.
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Western blot analysis of STEAP1 in human spleen tissue lysate with STEAP1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of STEAP1 in human spleen tissue with STEAP1 antibody at 2.5 μg/mL.

Documents

Further Information

Additional Names:
STEAP1 Antibody: STEAP, PRSS24, STEAP, Metalloreductase STEAP1, Six-transmembrane epithelial antigen of prostate 1
Application Note:
STEAP1 antibody can be used for detection of STEAP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
STEAP1 Antibody: The six-transmembrane epithelial antigen of prostate 1 (STEAP1) was the first member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. The normal function of STEAP is still uncertain; unlike other members of the STEAP family, STEAP1 does not promote iron or copper reduction or uptake and lacks the FNO-like reductase domain critical for activity. However, its expression is highly increased in multiple cancer cell lines, including prostate, bladder, colon, and ovarian cancers. Supporting this is evidence that STEAP1 peptides can be used to stimulate CD8+ T cells from healthy donors, enabling them to recognize STEAP1-positive human tumor cells, suggesting that STEAP1 may a potential target for cancer immunotherapy. At least three isoforms of STEAP1 are known to exist.
Background References:
  • Hubert RS, Vivanco I, Chen E, et al. STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumors. Proc. Natl. Acad. Sci. USA1999; 96:14523-8.
  • Ohgami RS, Campagna DR, McDonald A, et al. The Steap proteins are metalloreductases. Blood2006; 108:1388-94.
  • Ohgami RS, Campagna DR, Greer EL, et al. Identification of a ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Nat. Genet.2005; 37:1264-9.
  • Alves PM, Faure O, Graff-Dubois S, et al. STEAP, a prostate tumor antigen, is a target of human CD8+ T cells. Cancer Immunol. Immunother.2006; 55:1515-23.
Buffer:
STEAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (94%)
Immunogen:
STEAP1 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human STEAP1.

The immunogen is located within the last 50 amino acids of STEAP1.
NCBI Gene ID #:
26872
NCBI Official Name:
six transmembrane epithelial antigen of the prostate 1
NCBI Official Symbol:
STEAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
EAL24166
Protein GI Number:
51094921
Purification:
STEAP1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Cancer
SPECIFICITY:
This STEAP1 antibody does not cross-react with other STEAP proteins.
Swissprot #:
Q9UHE8
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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