TDP43 Antibody

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ProSci
Product Code: PSI-4285
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4285-0.02mg0.02mg£150.00
Quantity:
PSI-4285-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of TDP43 in L1210 cell lysate with TDP43 antibody at (A) 0.5 and (B) 1 μg/mL.
2 / 3
Immunocytochemistry of TDP43 in HeLa cells with TDP43 antibody at 5 μg/mL.
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Immunofluorescence of TDP43 in Hela cells with TDP43 antibody at 20 μg/mL.
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Western blot analysis of TDP43 in L1210 cell lysate with TDP43 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of TDP43 in HeLa cells with TDP43 antibody at 5 μg/mL.
Immunofluorescence of TDP43 in Hela cells with TDP43 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
TDP43 Antibody: ALS10, TDP-43, TDP43, TAR DNA-binding protein 43
Application Note:
TDP43 antibody can be used for detection of TDP43 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TDP43 Antibody: TDP43 was initially identified as a novel cellular protein that bound to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3'splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.
Background References:
  • Ou SH, Wu F, Garcia-Martinez LF, et al. Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs. J. Virol.1995; 69:3584-96.
  • Buratti E, Dork T, Zuccato E, et al. Nuclear factor TDP-43 and SR proteins promote in vitro and in vivo CFTR exon 9 skipping. EMBO J.2001; 20:1774-84.
  • Neumann M, Sampathu DM, Kwong LK, et al. Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Science2006; 314:42-3.
Buffer:
TDP43 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (94%)
Immunogen:
TDP43 antibody was raised against a 18 amino acid synthetic peptide from near the center of human TDP43.

The immunogen is located within amino acids 160 - 210 of TDP43.
NCBI Gene ID #:
23435
NCBI Official Name:
TAR DNA binding protein
NCBI Official Symbol:
TARDBP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 46 kDa

Observed: 46 kDa
Protein Accession #:
ABO32290
Protein GI Number:
130750552
Purification:
TDP43 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
SPECIFICITY:
At least two isoforms are known to exist for this protein; this TDP43 antibody only recongizes the longer of the two isoforms.
Swissprot #:
Q13148
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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