PTEN Antibody

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ProSci
Product Code: PSI-3515
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3515-0.02mg0.02mg£150.00
Quantity:
PSI-3515-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of PTEN in PC-3 cell lysate with PTEN antibody at 1 μg/mL in either the (A) absence or (B) presence of blocking peptide.
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Western blot analysis of PTEN in PC-3 cell lysate with PTEN antibody at 1 μg/mL in either the (A) absence or (B) presence of blocking peptide.

Documents

Further Information

Additional Names:
PTEN Antibody: BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1, PTEN1, 10q23del, Phosphatidylinositol 3, 4, 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Mutated in multiple advanced cancers 1
Application Note:
PTEN antibody can be used for the detection of PTEN by Western blot at 1 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
PTEN Antibody: PTEN (phosphatase and tensin homologue deleted on chromosome ten) is a dual-specificity phosphatase (with both protein and lipid phosphatase activity) first identified as a tumor suppressor gene. PTEN indirectly activates the AKT/PI3K pathway, an important signaling pathway for cell growth and proliferation by keeping levels of the second messenger PIP3 low, thereby preventing phosphoinositide-dependent kinase-1 (PDK-1) from phosphorylating and activating AKT. Expression of wild-type PTEN causes growth arrest in many cancer cell lines, but expression of a PTEN protein containing a mutation that blocks its lipid phosphatase activity failed to suppress growth of glioma cell lines suggesting that the tumor suppressive effect of PTEN is mediated solely by its lipid phosphatase activity. Other activities include the inhibition of insulin stimulated MAPK activation by blocking the insulin-receptor substrate (IRS)-1 phosphorylation and assembly of the IRS-1/Grb2/Sos complex.
Background References:
  • Li J, Yen C, Liaw D, et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science1997; 275:1943-7.
  • Steck PA, Pershouse MA, Jasser SA, et al. Identification of a candidate tumor suppressor gene, MMAC1, a chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat. Genet.1997; 15:356-62.
  • Wu X, Senechal K, Neshat MS, et al. The PTEN/MMAC1 tumor suppressor phosphatase functions as a negative regulator of the phosphoinositide 3-kinase/Akt pathway. Proc. Natl. Acad. Sci. USA1998; 95:15587-91.
  • Myers MP, Pass I, Batty IH, et al. The lipid phosphatase activity of PTEN is critical for its tumor suppressor function. Proc. Natl. Acad. Sci. USA1998; 95:13513-8.
Buffer:
PTEN Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PTEN antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human PTEN.

The immunogen is located within the last 50 amino acids of PTEN.
NCBI Gene ID #:
5728
NCBI Official Name:
phosphatase and tensin homolog
NCBI Official Symbol:
PTEN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
P60484
Protein GI Number:
42560209
Purification:
PTEN Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Cancer
Swissprot #:
P60484
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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