LIS1 Antibody
| Code | Size | Price |
|---|
| PSI-4287-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4287-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
LIS1 Antibody: MDS, LIS1, LIS2, MDCR, PAFAH, MDS, PAFAHA, Platelet-activating factor acetylhydrolase IB subunit alpha, Lissencephaly-1 protein, LIS-1
Application Note:
LIS1 antibody can be used for detection of LIS1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
LIS1 Antibody: Lissencephaly is a severe brain developmental disease characterized by the mislocalization of cortical neurons, a smooth cerebral surface, mental retardation, and seizures. Classical lissencephaly is caused by sporadic mutations in the LIS1 gene. While LIS1 is known to act in a pathway deactivating the lipid messenger platelet-activating factor, LIS1 forms a complex with Nudel and 14-3-3epsilon which is then transported from neuronal cell bodies through the actions of DISC1 and KIF5A, a microtubule-dependent directed motor protein kinesin. Decreased expression of LIS1 blocked neural stem cell division, morphogenesis, and motility, suggesting that LIS1 plays an important role in neuronal cell proliferation and localization in the developing brain. At least two isoforms of LIS1 are known to exist.
Background References:
- McManus MF and Golden JA. Neuronal migration in developmental disorders. J. Child Neurol.2005; 20:280-6.
- Reiner O, Carrozzo R, Shen Y, et al. Isolation of a Miller-Dieker lissencephaly gene containing G protein b-subunit-like repeats. Nature1993; 364:717-21.
- Hattori M, Adachi H, Tsujimoto M, et al. Miller-Dieker lissencephaly gene encodes a subunit of brain platelet activating factor. Nature1994; 370:216-8.
- Taya S, Shinoda T, Tsuboi D, et al. DISC1 regulates the transport of the NUDEL/LIS1/14-3-3e complex through kinesin-1. J. Neurosci.2007; 27:15-26.
Buffer:
LIS1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (100%), Chicken: (93%)
Immunogen:
LIS1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human LIS1.
The immunogen is located within amino acids 340 - 390 of LIS1.
The immunogen is located within amino acids 340 - 390 of LIS1.
NCBI Gene ID #:
5048
NCBI Official Name:
platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)
NCBI Official Symbol:
PAFAH1B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
P43034
Protein GI Number:
1170794
Purification:
LIS1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
P43034
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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