BRCC36 Antibody

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ProSci
Product Code: PSI-4331
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4331-0.02mg0.02mg£150.00
Quantity:
PSI-4331-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5 and (B) 1 μg/mL.
2 / 3
Immunohistochemistry of BRCC36 in human brain tissue with BRCC36 antibody at 2.5 μg/mL.
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Immunofluorescence of BRCC36 in Human Brain cells with BRCC36 antibody at 20 μg/mL.
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Western blot analysis of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of BRCC36 in human brain tissue with BRCC36 antibody at 2.5 μg/mL.
Immunofluorescence of BRCC36 in Human Brain cells with BRCC36 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
BRCC36 Antibody: C6.1A, BRCC36, CXorf53, C6.1A, Lys-63-specific deubiquitinase BRCC36, BRCA1-A complex subunit BRCC36
Application Note:
BRCC36 antibody can be used for detection of BRCC36 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
BRCC36 Antibody: Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.
Background References:
  • Dong Y, Hakimi MA, Chen X, et al. Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalsome-like subunit and its role in DNA repair. Mol. Cell2003; 12:1087-99.
  • Chen X, Arciero CA, Wang C, et al. BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation. Cancer Res.2006; 66:5039-46.
Buffer:
BRCC36 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (76%)
Immunogen:
BRCC36 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC36.

The immunogen is located within amino acids 20 - 70 of BRCC36.
NCBI Gene ID #:
79184
NCBI Official Name:
BRCA1/BRCA2-containing complex, subunit 3
NCBI Official Symbol:
BRCC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 35 kDa

Observed: 35 kDa
Protein Accession #:
NP_077308
Protein GI Number:
13236583
Purification:
BRCC36 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
P46736
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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