TEM7 Antibody

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ProSci
Product Code: PSI-4339
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4339-0.02mg0.02mg£150.00
Quantity:
PSI-4339-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of TEM7 in human liver tissue lysate with TEM7 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
2 / 3
Immunohistochemistry of TEM7 in mouse liver tissue with TEM7 antibody at 2.5 μg/mL.
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Immunofluorescence of TEM7 in mouse liver tissue with TEM7 antibody at 20 μg/mL.
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Western blot analysis of TEM7 in human liver tissue lysate with TEM7 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunohistochemistry of TEM7 in mouse liver tissue with TEM7 antibody at 2.5 μg/mL.
Immunofluorescence of TEM7 in mouse liver tissue with TEM7 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
TEM7 Antibody: TEM3, TEM7, TEM3, Plexin domain-containing protein 1, Tumor endothelial marker 3
Application Note:
TEM7 antibody can be used for detection of TEM7 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
TEM7 Antibody: Tumor endothelial marker 7 (TEM7) was identified through serial analysis of gene expression on endothelial cells isolated from human normal and malignant colorectal tissues. Further experiments verified TEM7 was highly expressed in the endothelium of numerous other cancer types including breast, lung and brain tumors. At least four isoforms of TEM7 are known to exist; these include intracellular, secreted, and membrane-bound forms. A homologous protein, TEM7R (also known as PLXDC2), acts as a binding partner to TEM7 and is also abundantly expressed in the endothelium of malignant colorectal cancer but is absent or rare in normal colon mucosa. High expression of TEM7 is associated with metastasis and poor survival of patients with osteogenic sarcoma.
Background References:
  • St. Croix B, Rago C, Velculescu V, et al. Genes expressed in human tumor endothelium. Science2000; 289:1197-202.
  • Nanda A, Buckhaults P, Seaman S, et al. Identification of a binding partner for the endothelial cell surface proteins TEM7 and TEM7R. Cancer Res.2004; 64:8507-11.
  • Carson-Weber EB, Watkins DN, Nanda A, et al. Cell surface tumor epithelial markers are conserved in mice and humans. Cancer Res.2001; 61:6649-55.
  • Fuchs B, Mahlum E, Halder C, et al. High expression of tumor endothelial marker 7 is associated with metastasis and poor survival of patients with osteogenic sarcoma. Gene2007; 399:137-43.
Buffer:
TEM7 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TEM7 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human TEM7.

The immunogen is located within the last 50 amino acids of TEM7.
NCBI Gene ID #:
57125
NCBI Official Name:
plexin domain containing 1
NCBI Official Symbol:
PLXDC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAG00869
Protein GI Number:
14017379
Purification:
TEM7 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q8IUK5
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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