MANF Antibody
Code | Size | Price |
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PSI-4347-0.02mg | 0.02mg | £150.00 |
PSI-4347-0.1mg | 0.1mg | £449.00 |
Overview
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Images
Documents
Further Information
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
- Shridhar et al. Oncogene1996; 12:1931-9.
- Shridhar et al. Cancer Res.1996; 56:5576-8.
- Petrova et al. J. Mol. Neurosci.2003; 20:173-88.
- Lindholm et al. Nature 2007; 448:73-7.
The immunogen is located within the last 50 amino acids of MANF.
Observed: 18 kD
KO Validation (Figure 1): Anti-MANF antibodies (4347) specificity was further verified by MANF specific knockout. MANF signal was not detected in MANF knockout HEK293T cells as compared to that in control wild type cells.
Recombinant Protein Test (Figure 3): Anti-MANF antibodies (4347) detected human MANF recombinant protein at different concentrations.
Localization Validation (Figure 8): MANF detected by anti-MANF antibodies is colocalized with HuC/D (a general neural marker). MANF is localized in mature neuronal cells.
KD Validation (Figure 9): Anti-MANF antibody (4347) specificity was further verified by MANF specific knockdown. MANF signal, when injected with a combination of two antisense splice-blocking morpholino oligonucleotides (MOsp), was disrupted in comparison with either wild type or when injected with control MO .
Induced Expression Validation (Figure 10): MANF expression detected by anit-MANF antibodies was up-regulated by VPA treatment.
References
- Chen et al. MANF regulates dopaminergic neuron development in larval zebrafish. Dev Biol. 2012;370(2):237-49. PMID: 22898306
- Almutawaa et al. Induction of neurotrophic and differentiation factors in neural stem cells by valproic acid. Basic Clin Pharmacol Toxicol. 2014;115(2):216-21. PMID: 24460582
- Niles et al. Valproic acid up-regulates melatonin MT1 and MT2 receptors and neurotrophic factors CDNF and MANF in the rat brain. Int J Neuropsychopharmacol. 2012;15(9):1343-50. PMID: 22243807
- Walaa S. Almutawaa. Induction Of Neurotrophic and Differentiation Genes in Neural Stem Cells by Valproic Acid. McMaster University, M.Sc. Thesis. 2012.PMID:
- Merja H. Voutilainen. CDNF and MANF in an experimental model of Parkinson's disease in rats. University of Helsinki, Ph.D. Thesis. 2010.PMID:
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