TEM1 Antibody

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ProSci
Product Code: PSI-4359
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4359-0.02mg0.02mg£150.00
Quantity:
PSI-4359-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 1 Western Blot Validation in Human Colon Tissue </strong><br> Loading: 15 μg of lysates per lane. Antibodies: TEM-1 4359, (A, 0.5μg/mL; B, 1μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TEM-1 4357, (2 μg/mL), TEM-1 4359, ( 1μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Human Tissue</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TEM-1 4359, (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Immunohistochemistry Validation of TEM-1 in Human Colon Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded Human Colon tissue using anti-TEM-1 antibody (4359) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 5 Immunofluorescence Validation of TEM-1 in Human Colon Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling TEM-1 with 4359 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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<strong>Figure 1 Western Blot Validation in Human Colon Tissue </strong><br> Loading: 15 μg of lysates per lane. Antibodies: TEM-1 4359, (A, 0.5μg/mL; B, 1μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TEM-1 4357, (2 μg/mL), TEM-1 4359, ( 1μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Tissue</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TEM-1 4359, (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of TEM-1 in Human Colon Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded Human Colon tissue using anti-TEM-1 antibody (4359) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunofluorescence Validation of TEM-1 in Human Colon Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling TEM-1 with 4359 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Documents

Further Information

Additional Names:
TEM1 Antibody: TEM1, CD164L1, TEM1, Endosialin, Tumor endothelial marker 1
Application Note:
WB: 0.5-2 μg/mL; IHC-P: 2.5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TEM1 Antibody: Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion.
Background References:
  • Rettig et al. Proc. Natl. Acad. Sci. USA 1992; 89:10832-6.
  • Christian et al. J. Biol. Chem. 2001; 276:7408-14.
  • Nanda?et al. Curr. Opin. Oncol. 2004; 16:44-9.
  • Nanda et al. Proc. Natl. Acad. Sci. USA 2006; 103:3351-6.
Buffer:
TEM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse (100%); Rat: (93%)
Immunogen:
Anti-TEM-1 antibody (4359) was raised against a peptide corresponding to 14 amino acids near the amino terminus of human TEM-1.

The immunogen is located within amino acids 40-90 of TEM-1
ISOFORMS:
Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD), and isoform 2 (433aa, 46kD). Mouse TEM-1 has one isoform (765aa, 82kD) and Rat TEM-1 also has one isoform (764aa, 82kD). 4359 can detect human isoform 1, mouse and rat TEM-1.
NCBI Gene ID #:
57124
NCBI Official Name:
CD248 molecule, endosialin
NCBI Official Symbol:
CD248
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 81kD

Observed: 90 kD (post-modifications: 27 O-linked glycosylations)
Protein Accession #:
NP_065137
Protein GI Number:
9966885
Purification:
TEM1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Cancer
SPECIFICITY:
At least two isoforms of TEM1 are known to exist; this antibody recognizes only the larger isoform.
Swissprot #:
Q9HCU0
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar TEM-1 expression profile in human and mouse cell lines detected by two independent anti-TEM-1 antibodies that recognize different epitopes, 4357 against C-terminus domain and 4359 against N-terminus domain.  TEM-1 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

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