Nhe-1 Antibody
| Code | Size | Price |
|---|
| PSI-4377-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4377-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Nhe-1 Antibody: APNH, NHE1, NHE-1, APNH1, APNH
Application Note:
Nhe-1 antibody can be used for detection of Nhe-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Nhe-1 Antibody: The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension.
Background References:
- Mendoza SA. The Na+-H+ antiport is a mediator of cell proliferation. Acta Paediatr. Scand. 1987; 76:545-7.
- Denker SP, Huang DC, Orlowski J, et al. Direct binding of the NA?H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation. Mol. Cell. 2000; 6:1425-36.
- Cingolani HE, Rebolledo OR, Portiansky EL, et al. Regression of hypertensive myocardial fibrosis by NA (+)/H(+) exchange inhibition. Hypertension 2003; 41:373-7.
Buffer:
Nhe-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (95%), Pig: (95%), Bovine: (95%)
Immunogen:
Nhe-1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human Nhe-1.
The immunogen is located within the last 50 amino acids of Nhe-1.
The immunogen is located within the last 50 amino acids of Nhe-1.
NCBI Gene ID #:
6548
NCBI Official Name:
solute carrier family 9 (sodium/hydrogen exchanger), member 1
NCBI Official Symbol:
SLC9A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 52, 90 kDa
Observed: 50, 90 kDa
Observed: 50, 90 kDa
Protein Accession #:
P19634
Protein GI Number:
127809
Purification:
Nhe-1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
SPECIFICITY:
At least three isoforms of Nhe-1 are known to exist.
Swissprot #:
P19634
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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