TOCA-1 Antibody
| Code | Size | Price |
|---|
| PSI-4375-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4375-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
TOCA-1 Antibody: TOCA1, C1orf39, TOCA1, Formin-binding protein 1-like, Transducer of Cdc42-dependent actin assembly protein 1, Toca-1
Application Note:
TOCA-1 antibody can be used for detection of TOCA-1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TOCA-1 Antibody: Actin reorganization is important for the regulation of neuronal morphology. A protein involved in this process, the transducer of cdc42-dependent actin assembly 1 (TOCA-1) protein, a member of the evolutionarily conserved pombe CDC15 homology (PCH) protein family, is an essential component of the Cdc42 pathway. TOCA-1 binds both N-WASP and Cdc42 and is essential for Cdc42- and PIP2-induced actin polymerization, suggesting that TOCA-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Decreased expression of TOCA-1 significantly enhances neurite elongation in PC-12 cells; its overexpression in the same cells suppresses neurite elongation. It has been suggested that TOCA-1 negatively regulates axon branching by regulating membrane trafficking by regulating membrane trafficking through the F-BAR/EFC domain. Multiple isoforms of TOCA-1 are known to exist.
Background References:
- Ho H-Y H, Rohatgi R, Lebensohn AM, et al. Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Cell2004; 118:203-16.
- Kakimoto T, Katoh H, and Negishi M. Regulation of neuronal morphology by Toca-1, an F-BAR/EFC protein that induces plasma membrane invagination. J. Biol. Chem.2006; 281:29042-43.
Buffer:
TOCA-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TOCA-1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of the human TOCA-1.
The immunogen is located within amino acids 40 - 90 of TOCA-1.
The immunogen is located within amino acids 40 - 90 of TOCA-1.
NCBI Gene ID #:
54874
NCBI Official Name:
formin binding protein 1-like
NCBI Official Symbol:
FNBP1L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001020119
Protein GI Number:
68348709
Purification:
TOCA-1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
Q5T0N5
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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