ATG10 Antibody
| Code | Size | Price |
|---|
| PSI-4399-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4399-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ATG10 Antibody: APG10, APG10L, pp12616, PP12616, Ubiquitin-like-conjugating enzyme ATG10, Autophagy-related protein 10, APG10-like
Application Note:
ATG10 antibody can be used for the detection of ATG10 by Western blot at 0.5 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
ATG10 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy protein family is ATG10, an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and the modification of a soluble form of MAP-LC3, a homolog of yeast Apg8, to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Multiple isoforms of ATG10 are known to exist.
Background References:
- Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906.
- Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.
- Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol.2000; 150:1507-13.
- Nemoto T, Tanida I, Tanida-Miyake E, et al. The mouse APG10 homologue, an E2-like enzyme for APG12p conjugation, facilitates MAP-LC3 modification. J. Biol. Chem.2003; 278:39517-26.
Buffer:
ATG10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ATG10 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ATG10.
The immunogen is located within the last 50 amino acids of ATG10.
The immunogen is located within the last 50 amino acids of ATG10.
NCBI Gene ID #:
83734
NCBI Official Name:
ATG10 autophagy related 10 homolog (S. cerevisiae)
NCBI Official Symbol:
ATG10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
EAW95884
Protein GI Number:
119616290
Purification:
ATG10 Antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
Swissprot #:
Q9H0Y0
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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