ZBP1 Antibody
| Code | Size | Price |
|---|
| PSI-4401-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4401-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ZBP1 Antibody: DAI, DLM1, DLM-1, C20orf183, Z-DNA-binding protein 1, Tumor stroma and activated macrophage protein DLM-1
Application Note:
ZBP1 antibody can be used for the detection of ZBP1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ZBP1 Antibody: Z-DNA binding protein 1 (ZBP1) belongs to a family of proteins that contain the Zalpha domain which binds specifically to left-handed Z-DNA and Z-RNA. ZBP1 was initially identified as a novel gene that was up-regulated in activated macrophages in mice bearing ascites tumors, suggesting that is may play a role in processes such as host response in neoplasia. More recent reports indicate that the cytosolic ZBP1 has can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of ZBP1 to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK).
Background References:
- Schwartz T, Behlke J, Lowenhaupt K, et al. Structure of the DLM-1-Z-DNA complex reveals a conserved family of Z-DNA-binding proteins. Nat. Struct. Biol. 2001; 8:761-5.
- Fu Y, Comella N, Tognazzi K, et al. Cloning of DLM-1, a novel gene that is up-regulated in active macrophages, using RNA differential display. Gene 1999; 204:157-63.
- Takaoda A, Wang Z, Choi MK, et al. DAI (DLM-1/ZBP1) is a cytosolic DNA sensor and an activator of innate immune response. Nature 2007; 448:501-5.
Buffer:
ZBP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ZBP1 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human ZBP1.
The immunogen is located within the last 50 amino acids of ZBP1.
The immunogen is located within the last 50 amino acids of ZBP1.
NCBI Gene ID #:
81030
NCBI Official Name:
Z-DNA binding protein 1
NCBI Official Symbol:
ZBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 39, 45 kDa
Observed: 42 kDa
Observed: 42 kDa
Protein Accession #:
EAW75510
Protein GI Number:
119595916
Purification:
ZBP1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
SPECIFICITY:
Multiple isoforms of ZBP1 are known to exist.
Swissprot #:
Q9H171
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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