CXCR4-Lo Antibody

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ProSci
Product Code: PSI-4443
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-4443-0.02mg0.02mg£150.00
Quantity:
PSI-4443-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of CXCR4 in (A) human spleen and (B) human thymus tissue lysate with CXCR4-Lo antibody at 10 μg/mL.
2 / 3
Immunohistochemistry of CXCR4Lo in HeLa cells with CXCR4Lo antibody at 2 μg/mL.
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Immunofluorescence of CXCR4-Lo in Hela cells with CXCR4-Lo antibody at 4 μg/mL.
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Western blot analysis of CXCR4 in (A) human spleen and (B) human thymus tissue lysate with CXCR4-Lo antibody at 10 μg/mL.
Immunohistochemistry of CXCR4Lo in HeLa cells with CXCR4Lo antibody at 2 μg/mL.
Immunofluorescence of CXCR4-Lo in Hela cells with CXCR4-Lo antibody at 4 μg/mL.

Documents

Further Information

Additional Names:
CXCR4-Lo Antibody: FB22, HM89, LAP3, LCR1, NPYR, WHIM, CD184, LESTR, NPY3R, NPYRL, HSY3RR, NPYY3R, D2S201E, C-X-C chemokine receptor type 4, FB22, CXC-R4
Application Note:
CXCR4-Lo antibody can be used for Western blot at 10 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
CXCR4-Lo Antibody: Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The alpha-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites.
Background References:
  • Dimitrov DS. Cell1997; 91:721-30.
  • Feng Y et al. Science1996; 272:872-7.
  • Berson JF et al. J. Virol.1996; 70:6288-95.
  • Doranz BJ et al. Cell 1996; 85:1149-58.
Buffer:
CXCR4-Lo Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CXCR4-Lo antibody was raised against a peptide corresponding to nine amino acids near the amino terminus of human CXCR4 isoform a.

The immunogen is located within the first 50 amino acids of CXCR4-Lo.
NCBI Gene ID #:
7852
NCBI Official Name:
chemokine (C-X-C motif) receptor 4
NCBI Official Symbol:
CXCR4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001008540
Protein GI Number:
56790927
Purification:
CXCR4-Lo Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease
SPECIFICITY:
This antibody is specific for the longer isoform of CXCR4.
Swissprot #:
P61073
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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