SCRAPPER Antibody
| Code | Size | Price |
|---|
| PSI-4451-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-4451-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
SCRAPPER Antibody: Fbl2, Fbl20, FBL2, F-box and leucine-rich repeat protein 20
Application Note:
SCRAPPER antibody can be used for detection of SCRAPPER by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SCRAPPER Antibody: Members of the F-box protein family, such as Scrapper, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. Scrapper is selectively expressed in the brain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane. Scrapper has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. Scrapper is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.
Background References:
- Ho MS, Tsai PI, and Chien CT. F-box proteins: the key to protein degradation. J. Biomed. Sci.2006; 13:181-91.
- Dobie F and Craig AM. A fight for neurotransmission: SCRAPPER trashes RIM. Cell2007; 130:775-7.
- Yao I, Takagi H, Ageta H, et al. SCRAPPER-dependent ubiquitination of active zone protein RIM1 regulates synaptic vesicle release. Cell2007; 130:943-57.
- Ding M, Chao D, Wang G, et al. Spatial regulation of an E3 ubiquitin ligase directs selective synapse elimination. Science2007; 317:947-51.
Buffer:
SCRAPPER Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
SCRAPPER antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of the human SCRAPPER.
The immunogen is located within the first 50 amino acids of SCRAPPER.
The immunogen is located within the first 50 amino acids of SCRAPPER.
NCBI Gene ID #:
84961
NCBI Official Name:
F-box and leucine-rich repeat protein 20
NCBI Official Symbol:
FBXL20
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_116264
Protein GI Number:
27734755
Purification:
SCRAPPER Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
Q96IG2
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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